Background: Sepsis still remains an important cause of morbidity and mortality posing a diagnostic challenge to clinicians across the globe. Lack of a single specific and sensitive test paved the way to many researches. Our study evaluated clinical significance of cell population data (CPD) as biomarkers in sepsis. CPD assesses morphological and functional characteristics of leucocytes using automated hematology analyzer. Methods:The study population consisted of 100 healthy subjects and 100 clinically suspected cases of sepsis with quick sequential organ failure assessment (qSOFA) score >2. The various cell population data (CPD) were collected using automated hematology analyzer, Sysmex XN1000 during a span of 6 months from January to June 2018 in a tertiary care center and the results were statistically analyzed using Z test. Result:The WBC count and CPD parameters were assessed. Except for neutrophil , monocyte cell size (NE-FSC, MO-Z) and lymphocyte fluorescence intensity(LY-Y) all other CPD parameters show significant difference (p<0.001) in sepsis group compared to healthy controls. Conclusion:This study suggested utility of CPD parameters which provide details of size and internal structure of leucocytes to be considered as an important biomarker for diagnosis and management of sepsis.
Nucleated red blood cells are immediate precursors of mature erythrocytes and are less deformable to pass through fenestrations in endothelial lining of bone marrow. Therefore, its presence in circulation i.e. normoblastemia after the neonatal period is considered abnormal and needs further evaluation. [1,2,4] Materials and MethodsThis retrospective study was conducted in a tertiary care centre. The study period was 3months, i.e. from February 2018 to April 2018. The presence of nRBCs in the peripheral blood was detected with the help of automated analyzer (Sysmex XN 1000) and by laboratory data retrieving. Peripheral smear correlation was done. It was followed by tabulation and analysis of results. Among the patients of age ( 15-25years ), the causes were microcytic hypochromic anemia ± neutrophilia (n=10), thrombocytopenia (n=1), eosinophilia (n=1) and pancytopenia (n=1). The count ranged from 0.1-1.5 nRBCs/100WBC.In patients of >25years of age, a total of (n=40) cases were microcytic hypochromic anemia, (n=24) were normocytic
Background: Myeloproliferative neoplasms (MPN) are clonal hematopoietic stem cell diseases characterised by myeloid cell growth from one or more lineages. Angiogenesis, in contrast to other subtypes, plays a substantial role in the pathophysiology of primary myelofibrosis (PMF). Research expressing the correlation of microvessel density (MVD), blasts, fibrosis and mast cell count in MPN cases are rarely conducted. We aimed to study the significance of MVD in correlation with CD34 blasts, mast cells and fibrosis in bone marrow biopsies of MPN patients. Methods: The current research was a cross sectional study conducted on 66 cases diagnosed as MPN during a six-year period. This comprised of 32 chronic myeloid leukemia (CML), 31 PMF and three essential thrombocythemia (ET) cases. Routine staining along with reticulin stain to look for fibrosis and immunohistochemistry (IHC) using CD34 and mast cell tryptase (MCT) were performed. Results: We found increased MVD in PMF, when compared to CML and ET (p = 0.042). Further, mean MVD was observed to be increased with high blast counts (p = 0.036). On follow up, raised mean MVD was seen in those cases with relapse/deceased as compared to disease-free patients, which was highly significant (p = 0.000). Conclusions: Increased MVD score was mostly associated with PMF subtype among all the MPNs. Further, higher MVD was observed to be associated with increased blast count and poor prognosis. With angiogenesis playing a critical role in disease outcome, we now have drugs to regulate angiogenesis that are supported by contemporary research. However, further studies with larger cohorts to establish the theranostic role of MVD in MPNs is recommended.
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