The generation of machining defects and their effect on bending strength characteristics are discussed with respect to a TiAl intermetallic compound, which is attracting much attention as a high-temperature structural material because of its high specific strength. The discussion focuses on the mechanism of the formation of edge chipping and machined surface cracks during cutting of a TiAl intermetallic compound. Also, some effects of these machining defects on the bending strength are clarified. The comparison of cutting results with abrasive machining results helps clarify the dependence of strength on undeformed chip thickness during chip generation of machining processes.
During large scale preparation of recombinant human basic fibroblast growth factor (hbFGF) mutein CS23(CS23) produced in Escherichia coli (E. coli), three species, named Mf-1, Mf-2 and Mf-3 in the order of their elution, were isolated from the early fraction of Sulfated Cellulofine chromatography by high performence liquid chromatography (HPLC) on heparin-5pw. Structural analysis through HPLC peptide mapping, mass spectrometry, sequencing and amino acid composition revealed that epsilon-N-acetylation occurred at positions Lys135, Lys26 and Lys119 of CS23. Together with this observation, the results on large scale preparation of CS23 were also presented.
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