Background: Gastroenteritis is a major public health problem worldwide and remains the second leading cause of death among children under five globally. Objective of this study was to investigate and compare the efficacy of commonly available probiotics in the treatment of acute watery diarrhoea (AWD) in children.Methods: This was an open label randomised controlled trial conducted in a tertiary care hospital in Bangalore for 2 years. 120 children aged 6 months to 5 years admitted with AWD of less than or equal to 48 hours duration were randomized into three groups – Group I (control) received oral rehydration therapy and zinc, Group II received Bacillus clausii in a dose of 2 billion spores twice a day and Group III received Saccharomyces boulardii as 250 mg twice a day. The primary outcome measures were total duration of diarrhoea, mean number of stools per day, consistency of stools and secondary outcome measures were duration of vomiting, fever and hospital stay. ANOVA, Student t test, Mann Whitney U test and Chi square test were used for analysis.Results: The duration of diarrhoea and hospital stay significantly reduced (41.68 hrs) in Group III compared to Group I (57.65 hrs) and Group II (53.33 hrs). (p< 0.05). The frequency of stools reduced significantly on Day 4 and the consistency of stools improved significantly on Day 3 in both the probiotic groups (p<0.05). Both the probiotics reduced the duration of fever significantly but had no effect on the duration of vomiting.Conclusions: Saccharomyces boulardii is effective in reducing the duration of diarrhea and hospital stay in children with acute gastroenteritis.
Background: Kangaroo mother care (KMC) is a practical low-cost intervention which is very cost effective in developing countries for babies weighing less than 2500g. This study aimed to evaluate the knowledge, attitude and practice of post-natal mothers of LBW babies after giving a teaching session by trained personnel in a tertiary care center in Karnataka.Methods: A cross sectional descriptive study of 90 mothers whose neonates were weighing less than 2.5kg from May to October 2018.Mothers were interviewed at enrolment and were assessed about their knowledge and were oriented on its practice.Results: At recruitment (95.5%) mothers did not know about KMC.80% of mothers followed in first sitting and 86.6% of the mothers felt photo exhibition was better than verbal.96.6% of mothers thought KMC is beneficial and it was believed that other family members can even provide KMC.Conclusions: Maternal knowledge was low before now they are happily practicing KMC in hospital and thereafter at home.
Mycobacterium tuberculosis is a leading cause of human mortality worldwide and the emergence of drug-resistantstrains, demands the discovery of new classes of antimycobacterials that can be employed in the therapeutic pipeline. Previously, a secondary metabolite Chrysomycin A, isolated from Streptomyces sp. OA161 was shown to have potent bactericidal activity against drug-resistant clinical isolates of M. tuberculosis and different species of mycobacteria. The antibiotic inhibits the mycobacterial topoisomerase I and DNA gyrase leading to bacterial death, but the mechanisms that could cause resistance are currently unknown. To further understand the resistance mechanism, spontaneous resistance mutants were isolated and subjected to whole-genome sequencing. Mutation in a TetR family transcriptional regulator MSMEG_1380 was identified in the resistant isolates and was close to an operon encoding membrane protein MSMEG_1381 and MSMEG_1382. Sequence analysis and modeling studies indicated that they are components of the Mmp family of efflux pumps and over-expression of either the operon or individual genes conferred resistance to chrysomycin A, isoniazid, and ethambutol that are in TB therapy. Our study highlights the role of membrane transporter proteins in conferring multiple drug resistance and the utility of recombinant strains overexpressing membrane transporters in the drug screening pipeline.
Background: Neonatal sepsis is a frequent and important cause of morbidity and mortality which accounts for one quarter of neonatal deaths. There are very few studies done in India to evaluate the role of MPV as diagnostic marker of neonatal sepsis.Methods: Prospective case control study in a tertiary care hospital. Neonates > 30 weeks gestation admitted to neonatal intensive care unit during the study period of 1 year with clinically suspected were included in the study. Neonates with Septic screen positive and culture positive sepsis were included in group A and normal neonates were included in Group B. MPV was done for all the subjects and values more than 10.2fl was considered positive. Newborns with congenital anomalies and who were already on antibiotics prior to admission were excluded from the study. Statistical analysis was done using Statistical Package of Social Sciences (SPSS) version 17.0.Results: 106 neonates were included in the study. MPV showed statistically significant difference between the study groups (mean 12.8±1.52, 10.82±1.20 respectively) at a cut of value of 10.2fl and a sensitivity of 93%, specificity of 84 % with a positive predictive value of 83% and negative predictive value of 94%.Conclusions: MPV can be used as an adjuvant marker along with established septic screen to ensure early diagnosis and treatment of neonatal sepsis with no additional expense.
Background: The aim of the study was to do universal hearing screening of all newborns using otoacoustic emission (OAE), to know the incidence and risk factors of hearing loss in neonates.Methods: This was a prospective observational study done in a tertiary care hospital in Mangalore city in Karnataka. 950 neonates were screened with distortion product otoacoustic emission (DPOAE) during the study period of one year from 2017 to 2018. A repeat test was done at one and a half months of age if the first test failed. Auditory brain stem evoked response (ABER) was performed at 3 months of age if both the tests failed. Babies with hearing loss were referred to ENT specialist for further management. Comparison of the variables was done by student’s t test and Chi-square test. P-value <0.05 was considered statistically significant.Results: Out of the 950 newborns screened with DPOAE test, 204 (21.4 %) babies had abnormal screen either in single or both ears. 7 out of 204 (3.43%) babies had abnormal OAE on repeat testing at one and half months. 2 out of 7 babies (0.96 %) had significant hearing loss ABER was performed at 3 months of age.Conclusions: Early identification by screening of hearing loss prevents a significant public health concern. Early recognition and intervention prior to 6 months have a significant positive impact on development.
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