Background and Aim Genetic factors are vital participants in the development and progression of myocardial infarction (MI). Adiponectin has been assumed to have a protective role in MI and adiponectin receptors variants could be a determinant for atherosclerosis. We aimed to evaluate the prevalence of ADIPOQ (rs2241766) and ADIPOR2 (rs10773989) polymorphisms and their association with mRNA levels and circulatory adiponectin levels in patients with MI. Subjects and Methods A total of 220 participants were classified into two groups: group 1 included 120 patients with MI, and group 2 involved 100 healthy participants as controls. Genotyping of ADIPOQ (rs2241766) and ADIPOR2 (rs10773989) polymorphisms were analyzed using an allele discrimination assay with real-time PCR and their relative expression or mRNA levels were determined by real-time PCR. Serum adiponectin level was determined using an ELISA technique. Results The ADIPOQ rs2241766 GG genotype and G allele and the CC genotype and C allele of ADIPOR2 rs10773989 were significantly prevalent in patients with MI and associated with increased risk of MI. We detected a marked reduction in serum adiponectin, ADIPOQ and ADIPOR2 mRNA levels in patients than control. The GG genotype of ADIPOQ rs2241766 and the CC genotype of ADIPOR2 rs10773989 had the lowest levels of their mRNA and adiponectin level in both patients and controls. Conclusion Adiponectin gene and receptor variants are potentially related to MI risk; furthermore, their expressions were markedly depressed in MI which suggests their use as potential biomarkers for MI.

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Background About 30% of cases with psoriasis will suffer from psoriatic arthritis (PsA). Heritable element plays a role in PsA as different genes are involved. However, few genes are involved in both psoriasis and PsA. This study aimed to investigate the predictive value of Toll-like receptor (TLR) 4 and colony stimulating factor (CSF) 2 gene expression for early detection of axial spondyloarthritis in psoriatic patients. Methods This study included 200 subjects; 100 psoriatic patients, subdivided into two groups; Group 1: included 66 patients with plaque psoriasis without any articular complaint, and Group 2: included 44 patients with psoriatic arthritis. Group 3 included: 100 age and sex matched healthy controls. Laboratory assessment of TLR4 and CSF2 gene expression by real time polymerase chain reaction technique, and axial joint radiological assessment by Magnetic Resonance Imaging. Results There were significant increase of CSF2 and TLR4 gene expression levels in cases compared with controls (p < 0.001) for both. Additionally, a significant rise of CSF2 and TLR4 gene expression levels in cases with psoriatic arthritis compared to cases with psoriatic skin affection only (U = 2.45, p = 0.01, 3.34, p = 0.001 respectively. Receiver operating characteristic curve done for earlier detection of sub-clinical changes of axSpA regarding positive MRI results in cases with psoriasis and PsA respectively with P value < 0.001. Conclusion TLR4 and CSF2 gene expression have strong predictive value in early detection of axial SpA changes in asymptomatic and non-radiographic psoriatic patients which is equivalent and equal to the MRI predictive value.

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