This paper were for witness of "Ablution (Wudu) health benefits (HBs) through comparison nasal-cavity (NC) bacterial-content (BC) with gold-standard (GS) at high-altitude (HA) area, Taif, KSA", the mean of total results NC-BC produced 66.2% quantity, after GS inhalation the NC-BC decreased to 21.4%, while after ablution decreased to 29.4%. The bacterial Spp were isolated (Staph. aureus, CNS, Staph. epidermidis, Non haemolytic Strept. and E. coli). The most important bacteria are isolated from NC were in descending gradient as follow: (Staph. aureus, Staph. epidermidis, E. coli, CNS and Non haemolytic Strept.) were in (32, 30, 4, 3 and 3%) respectively. After use of the GS, the reminded bacteria were (Staph. aureus and Staph. epidermidis) were in (5 and 10%) respectively. While after ablution the results were recorded (Staph. aureus and Staph. epidermidis) were in (11 and 16%). The concluded an importance of ablution and use of each step well to maintain individuals hygiene, five times ablution is best cleaning the NC well during the day. Where the results confirm the oozing of a healthy result and protection against bacterial infectious diseases and their bacterial causes. This emphasized the need for good ablution for every step and continuous regular five times dally, this is from Islam rules for ablution and prayer, which is the pillar of Islam for the person. That recommends regular ablution five times and works every step accurately as requested Islam and regular five times a day to gain the benefits of ablutions, especially in NC.
This work was done for estimation the degree of losing the antimicrobial activity for fresh and dry Ginger (Zingiber officinale) due to dryness proses and shelf life time stores. The experiments were done by exposure of some pathogenic microorganisms (MOs) to Ginger boil water extracts and detection of mortality rates of MOs.
A bortion is the most serious and economical issue disturbs the reproductive efficacy leading to a decrease in milk production, replacement of herd, expensive care and a rise in culling rate (Tulu et al., 2018). Several bacterial agents such as brucella, listeria, leptospira species, and viral agents such as BVD, IBR as well as mycotic and protozoal agents are the major infectious causes of bovine abortion. In addition, genetic, management, environmental and geographical factors are also included in non-infectious determinants (Tulu et al., 2018). Abortion typically takes place between 42 and 260 days of pregnancy (Peter, 2000). More than 2-5% of abortion rate is considered research Article Abstract | Abortion is one of the devastating and economic problems in dairy sector. This study aimed to determine the common bacterial cause of bovine abortion in Menoufiya governorate, Egypt from October 2018 till December 2019. Out of examination of 600 cows, 100 aborted cases (16.7%) were observed during this period and 55 vaginal swabs, 15 fetal stomach contents and 30 milk samples were collected from aborted cows and subculture onto specific medium. Our findings concluded that bacterial isolation reported that B. melitensis and L. monocytogenes were the most isolated from 55 vaginal swabs with 3.6% followed by M. bovis 1.8%. Meanwhile B. abortus and B. melitensis were the most common with 13.3% from 15 foetal stomach content followed by L. monocytogenes 6.6%. Moreover, M. bovis and B. abortus, and L. monocytogenes were isolated from 30 milk samples with 6.6%. The PCR results revealed successful amplification of B. abortus, B. melitensis, M. bovis and L. monocytogenes at 498, 731, 360, and 938 respectively. The sequencing analysis of B. melitensis, B. abortus, L. monocytogenes and M. bovis demonstrated identical and strong similarities (96.67-100%) with many international field strains in the Gene bank. To the best of our knowledge, this was the first report for L. monocytogenes and M. bovis from aborted fetus that showed high homologues with many international field stains. In conclusion, B. melitensis, B. abortus, M. bovis and L. monocytogenes, were the most common bacterial causes of bovine abortion in Egypt, thus promoting more attention and studies to establish new methods for control measures.
Listeria monocytogenes from Listeria genus, which as saprophytes, but causes systemic infections as pathogenic bacteria. Listeria monocytogenes is widely spread in environment, caused infection of farm animals during fields grazing leading to contamination by wildlife, or manure. There was a close relation between improperly farm environment originated from contaminated crops and listeriosis. It causes animals infection with is not associated with clinical symptoms then the animal is flaking Listeria monocytogenes in feces. Listeria species' identification is a serious to gain the ecological Listeria virulence influence. The samples understudy consisted of animal stool, mattress, ration and water (n = 30) were for each. All samples were exposed to bacterial check. The results obtained that Listeria species was isolated with a general occurrence of 33.3%. The stools had 17.5%, mattress 7.5%, ration 5.0%, and water 3.3%. Meanwhile the occurrence of Listeria monocytogenes was overall 20.0% included (10.0%, 5.0%, 2.5%, and 2.5 %) as the same aforementioned samples. The PCR-technique was applied for detection of Listeria monocytogenes virulence genes using primers groups. Those genes were hlyA (hemolysin A gene), inlA (internalin A), prfA. The results obtained the present of all virulence genes. Listeria monocytogenes susceptibility to antimicrobial agents usually used for farms treatment was determined for animal stools Listeria monocytogenes isolates. The results showed that the generally sensitive rate was 70.0%. The100.0% was sensitive to Ampicillin, Amoxicillin, Erythromycin, and Tetracycline followed by Ofloxacin 75.0%. But the 50.0% was sensitive to Penicillin G, Cephalexin, and Gentamycin, finally Lincomycin الزراعية للعلوم العربية المجلة مج( 5 )،ع ( 55 ) يوليو 2222 م 115 3 was resistant. It was concluded that the Listeria monocytogenes were isolated from understudy samples as noticeable degrees. Also, virulence genes were presented in the isolated Listeria monocytogenes. That powerful proved the attendance of Listeria monocytogenes trendy a hidden contamination in animal farm. So, that could affect the animal health and animal productions. It was recommended that the undertake to follow the "Farm Health Conditions" and follow-up to decrease the microbial contamination. As well fix the animal vaccinations to maintain the animal health and livestock.
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