Out of site: A protein expression system was combined with CuI‐catalyzed 1,2,3‐triazole formation to modify a target protein at its C terminus. The immobilized core protein can be modified by diverse small molecules in a site‐specific manner (see picture). Protein conjugation with a diazido linker gives a homodimeric protein, and microarrays can be fabricated by site‐specific covalent binding to functionalized surfaces.
The Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition has been demonstrated to be an effective and orthogonal conjugation reaction to covalently immobilize biomolecules on magnetic nanoparticles (MNPs). The azido group on the MNP surface provides better conjugation efficiency with alkynated molecules. Moreover, the C-terminal alkynated protein was site-specifically immobilized on MNP. The protein binding activity presented by site-specific immobilization is higher than that by random amide bond formation.
Gezielt ligiert: Ein Proteinexpressionssystem wurde mit der CuI‐katalysierten Bildung von 1,2,3‐Triazolen kombiniert, um ein Protein an seinem C‐Terminus zu modifizieren. Das immobilisierte Stammprotein lässt sich mit kleinen Molekülen ortsspezifisch ligieren (siehe Bild), wobei die Ligation mit einem Diazido‐Linker zu homodimeren Proteinkonjugaten führt. Durch spezifische kovalente Anbindung an funktionalisierte Oberflächen können außerdem Mikroarrays erzeugt werden.
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