In order to find the strains which can produce high ethanol yield as well as tolerate inhibitors on the lignocellulosic hydrolysates for developing the renewable bioenergy, the sepecial yeast must be explored. After acclimatizing 23 days and using five different acclimation media with sequential increase in the concentration of inhibitory compounds , a kind ofsaccharomyces cerevisiaestrain resistant to inhibitors was obtained . When the yeast resistant to drug and the parent strain grew in the same media which contained several inhibitory compounds 3.2 g/L acetic acid , 0.8 g/L furfural , 0.4 g/L formic acid , the new yeasts maximal ethanol yield can reach 0.428 g/g , up to 85.6% of theoretical ethanol yield. Compared with drug resistant yeast , the parent strains maximal ethanol production yield only can reach 0.246 g/g , up to 52.8% of theoretical ethanol yield . After 5 continuous ages , the average ability of producing ethanol was stable. Compared with parent strain, the yeast resistant to drug had good ability to ferment glucose and produce ethanol as well as tolerate inhibitors .The new yeast has extensive application prospect in the bioethanol production.
Biomass energy is environmentally friendly, renewable, and a rich stock.The research and development of biomass energy have become a hot spot. The β-glucosidase is the key enzyme in the utilization of biomass. The fermentation conditions for β-glucosidase production fromAspergillus nigerwere optimized. The optimum conditions were as follows: the seed age 48h, the inoculation amount 10%, the medium volume 20mL / 250mL, the initial pH 5.0, the rotational speed 200 r/ min, Under these conditions, the activity of β-glucosidase can research 13.92 U ∕ mL after 168h, increasing 92.8% than before. This experiment can provide the basis for the next step of liquid fermentation for the scaling up production of β-glucosidase.
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