View related articlesView Crossmark data Citing articles: 9 View citing articles Purification, characterization, and inhibition sensitivity of peroxidase from wheat (Triticum aestivum ssp. vulgare
In this study, a cationic soluble peroxidase (POD) was homogeneously purified and biochemically characterized. The enzyme purification involved the combination of (NH 4 ) 2 SO 4 precipitation, CM-Sephadex cation exchange, and gel filtration chromatography. The purity of the enzyme was examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and one single prominent band was obtained with a molecular weight of approximately 45 kDa. The thermal and pH stability showed that the enzyme was stable at pH 5.0 and at 40°C, respectively. The activity remained stable at pH 4.0 at least for 10 days. K m and V max values were calculated from Lineweaver-Burk graph for each substrate. POD exhibited K m values of 0.0154 and 0.065 mM for guaiacol and H 2 O 2 , respectively. The enzyme was highly inhibited by sodium azide. This study could enrich the literature regarding the properties of POD from haricot bean that could be applicable to biomedical analysis.
ARTICLE HISTORY
Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health (antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular). Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform (OAC), ethanol (OAE), and water (OAW) extract of Onobrychis argyrea subsp. argyrea (O. argyrea) specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71%, A549 92.14%, and HT-29 72.24% were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition (IC50: 34.12±0.2 and 21.58±0.12, respectively). OAE (257.95±1.40) and OAW (282.14±0.83) extracts showed
high activity with similar results in the FRAP method. Total phenolic content was determined as 226.15±1.89 mg GAE/g for OAE and 146.52±0.71 mg GAE/g for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich
secondary metabolites.
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