ABSTRAKDiet tinggi lemak, meningkatkan resiko aterosklerosis akibat stress oksidatif LDL yang di tunjukkan dengan peningkatan petanda inflamasi Adipocyte Fatty Acid Binding Protein ( A-FABP ). Quercetin, senyawa flavonoid mempunyai potensi antioksidan. Penelitian dilakukan untuk membuktikan efek pemberian quercetin terhadap penurunan serum A-FABP. Desain post test only control group dilakukan dengan hewan coba tikus wistar jantan yang di bagi dalam 2 kelompok control dengan diet normal dan 1% methylcellulose group, diet tinggi lemak dengan 1% methylcellulose, dan tiga kelompok perlakuan dengan diet tinggi lemak dan pemberian quercetin dosis 2mg/kgBB, 10mg/kgBB, quercetin 50mg/kgBB. Berbeda dengan hipotesis uji pearson menunjukkan hubungan korelasi positif peningkatan dosis quercetin dengan AFBP serum concentrations ( p<0.05, r=0,97). Kata Kunci : Adipocyte -Fatty Acid Binding Protein, diet tinggi lemak, quercetin ABSTRACT High Fat Diet (HFD) increase the risk of atherosclerosis due to oxydative stress of LDL indicated by elevation of Adypocyte Fatty Acid Binding Protein an inflamatory marker. Quercetin, a flavanoid compund, has been assumed as a potential antioxidant. This study aimed to investigate the impact between quercetin dose and decreasing of A-FABP serum concentration.A post test only control group using male wistar rats divided into two control groups which are 1) normal diet with 1% methylcellulose group, 2) high fat diet with 1% methylcellulose, and three treatment groups which are high fat diet groups with 2 mg/kgBB, 10mg/kgBB, quercetin 50mg/kgBB. Pearson correlation test showed the positive correlation betwen quercetin dose and AFBP serum concentrations ( p<0.05, r = 0,97 ).
The food processing by heating can increase the formation of AGEs (Advanced Glycation End Products). AGEs are compounds that formed from a non-enzymatic continuous glycation reaction between proteins and sugar residues. The CML (N-carboxymethyl-lysine) is used as a marker for AGEs cause most commonly found in vivo. The bond of AGEs and RAGE (reseptor for AGEs) induce various signaling pathways that trigger inflammation and increase oxidative stress. The AGE and RAGE interaction activate the transcription factor of NF-κβ. NF-κβ activate gene transcription to release proinflammatory cytokines such as TNF-α. Anthocyanins are compounds that can prevent the formation of AGEs and muffle the adverse effects of AGEs. Rosella contains anthocyanin such as : delphinidin-3-O-glucoside, delphinidin-3-Osambubioside, and cyanidin-3-O-sambubioside. This study is to determine whether the daily intake of Rosella extract can reduce the levels of n-carboxymethyl-lysine in serum, expression of NF-κβ and TNF α in the rats fed with heated food. This study applied experimental post test control using Rattus norvegicus Wistar strain. The samples were divided into 5 groups: KN (negative control/fed without heating), KP (positive control/fed heated food but not treated by Rosella extract), R1 (fed heated food and treated by 200 mg.kg-1 BW Rosella extract), R2 (fed heated food and treated by 300 mg.kg-1 BW Rosella extract), and R3 (fed heated food and treated by 400 mg.kg-1 BW Rosella extract). The ncarboxymethyl-lysine levels were measured by using the ELISA, the expression of NF-κβ is analyzed by using immunofluorescence, and expression of TNF-α is observed by immunohistochemistry. There was significantly decreased the levels of n-carboxymethyl-lysine in all groups which were treated by Rosella extracts (R1,R2,R3); p= 0.000, α = 0.05 (p< α). Decreased activation of NF-κβ in all groups which were treated by Rosella extract is significant (p = 0.000), and decreased expression of TNF α in all groups which were treated by Rosella extract is also significant (p = 0.000). Rosella extract can reduce the levels of n-carboxymethyl-lysine, expression of NF-κβ, and TNF α.
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