Denervation of the colon is protective against the colon cancer; however, the mechanisms involved are unknown. We tested the hypothesis that the denervated colonic mucosa could be less responsive to the action of the chemical carcinogen dimethylhydrazine (DMH). Three groups of 32 male Wistar rats were treated as follows: group 1 (G1) had the colon denervated with 0.3 mL 1.5 mM benzyldimethyltetradecylammonium (benzalkonium chloride, BAC); G2 received a single ip injection of 125 mg/kg DMH; G3 was treated with BAC + the same dose and route of DMH. A control group (Sham, N = 32) did not receive any treatment. Each group was subdivided into four groups according to the sacrifice time (1, 2, 6, and 12 weeks after DMH). Crypt fission index, ß-catenin accumulated crypts, aberrant crypt foci, and cell proliferation were evaluated and analyzed by ANOVA and the Student t-test. G3 animals presented a small number of aberrant crypt foci and low crypt fission index compared to G2 animals after 2 and 12 weeks, respectively. From the second week on, the index of ß-catenin crypt in G3 animals increased slower than in G2 animals. From the 12th week on, G2 animals presented a significant increase in cell proliferation when compared to the other groups. Colonic denervation plays an anticarcinogenic role from early stages of colon cancer development. This finding can be of importance for the study of the role of the enteric nervous system in the carcinogenic process.
O objetivo do estudo foi identificar e descrever as não conformidades encontradas nas fases préanalítica, analítica e pós-analítica de um Laboratório Público de Análises Clínicas. Para isso, com o uso de planilhas setorizadas e por um período de dez meses, diariamente foram anotadas as não conformidades identificadas desde a chegada do paciente portando a solicitação de exames até a conferência e liberação dos resultados dos mesmos para as Unidades de Saúde. Após o estudo realizado, concluiu-se que as não conformidades mais observadas na fase préanalítica foram a ocorrência de hemólise e casos de amostras não colhidas ou insuficientes, na fase analítica foram a contaminação de amostras, lipemia e a necessidade de repetir testes para confirmar resultados, e na fase pós-analítica foram a digitação incorreta e a necessidade de refazer testes para confirmar resultados. Anormalidades que podem ser sanadas mediante acompanhamento e treinamento constantes da equipe.
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