The aim of this study is to develop a green procedure that provides high‐efficiency extraction of phenolic compounds from Capparis Ovata fruit with Deep Eutectic Solvent (DES) using ultrasound‐assisted extraction method. Ten DES combinations were prepared by mixing various proportions of choline chloride (ChCl), Na‐acetate and Na‐citrate with sugar alcohols, carboxylic acid. These DESs were tested as an effective solvent for recovering phenolic compounds. DES composed of lactic acid combining with ChCl showed the highest efficiency of extraction for phenolic compounds. Optimal extraction conditions were found as follows; 20% water in DES, liquid/solid ratio 20:1 (mL/g), temperature 50℃, and extraction time 30 min. The maximum total phenolic content, total flavonoid content, DPPH inhibition percentage, and ferric reducing power capacity values under optimal conditions were 30.55 ± 0.57 mg GAE/g DW, 13.04 ± 0.4 mg QE/g DW, 98.49 ± 2.51% and 1.79 ± 0.04 (absorbance units), respectively. The concentrations of all phenolic compounds determined by chromatographic method have increased under optimal extraction conditions.
Practical applications
The application of DES in the extraction of the phenolic compound is an innovative approach for environmentally, friendly, low‐costs, and improving extraction efficiency. Caper fruits are known to be rich sources of bioactive compounds. Caper is consumed as food and traditional folk medicines for their health benefits. At previous studies, a green extraction procedure to obtain phenolic compounds from Capparis Ovata var canescens fruit has not been established by using DES. Therefore, this study aimed to improve a green procedure to obtain phenolic compounds with strong antioxidant properties, which could be employed in the food industry. In addition, determination of optimal extraction conditions has been performed to guide large‐scale production.
Negative attitudes towards the elderly may cause decreases in quality health service provided to the elderly. In the study, the aim was to determine attitudes of students studying in health related departments towards the elderly and relationships between the attitudes and various variables were analyzed. In a descriptive study, the sample included nursing, physiotherapy, and elderly care students. Kogan's attitude towards old people scale (KAOP) was used to measure attitudes towards the elderly and Stanley Coopersmith Self Esteem Inventory (SEI) was used to assess the level of students' self-esteem. The KOAP mean attitude score of the students was 125.6 ± 14.38 and the students had slightly positive attitudes towards the elderly. Students who were at the age of 20 or over and who were living in the city showed more positive attitudes. The students of the Elderly Care Department had the lowest mean score and there was a significant difference between mean KAOP scores of students at Nursing and Elderly Care Department. Also, weak positive correlation was found between the KAOP and SEI mean scores of students. The results implies that the students are required to enhance their positive attitudes towards the elderly. Therefore, students should be provided a training program that improves the positive attitudes.
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