<p>Kolom monolith berbasis polimer organik poli-(lauril metakrilat-co-etilen dimetakrilat) disintesis secara in situ kopolimerisasi dalam kolom <em>silicosteel </em>dengan ukuran panjang 10 cm dan diameter dalam 1,02 mm. Kolom monolith ini digunakan untuk pemisahan alkilbenzena secara Kromatografi Cair Kinerja Tinggi (KCKT) fasa terbalik. Pada penelitian ini, efisiensi pemisahan ditingkatkan dengan menggunakan kolom monolith poli-(LMA-co-EDMA) untuk memisahkan senyawa alkilbenzena melalui tiga parameter, yakni temperatur kolom, pemisahan secara isokratik dan pemisahan secara gradien. Temperatur kolom yang digunakan berkisar antara 27–50 °C. Hasil yang diperoleh menunjukkan bahwa temperatur optimum untuk pemisahan alkilbenzena secara isokratik yaitu 27 °C yang setara dengan temperatur ruang dengan fasa gerak asetonitril-air (50:50 w/w). Pemisahan alkilbenzena yang lebih efisien ditunjukkan dari penggunaan mode gradien ditandai dengan nilai <em>peak capacity</em>, faktor retensi dan jumlah plat teoritis yang lebih baik. Fasa gerak yang digunakan pada pemisahan secara gradien yaitu pelarut A yang terdiri atas asetonitril-air (40:60 w/w) dan pelarut B yang terdiri atas asetonitril-air (60:40 w/w) dengan waktu gradien 20–40 menit 0–100% B. Perubahan waktu gradien berpengaruh terhadap faktor retensi dan <em>peak capacity</em>.</p><p><strong>Evaluation of Organic Polymer-Based Monolithic Column by High Performance Liquid Chromatography for The Separation of Alkyl Benzenes</strong><strong>. </strong>Organic polymer-based monolithic column of poly(lauryl methacrylate-co-ethylene dimethacrylate) has been prepared by in- situ copolymerization inside of <em>silicosteel</em> column with the size of 100 mm long x 1.02 mm i.d. This kind of monolith column used for separation of alkylbenzenes using reversed-phase high performance liquid chromatography (HPLC). The efficiency separation on this research is improved by using poly-(LMA-co-EDMA) monolithic column for separation of alkyl benzene compounds using three strategies involving optimization column temperature, isocratic elution mode, and gradient elution mode. The applied column temperatures were varied in the range of 27–50 °C. It was found that room temperature in isocratic mode with the mobile phase of acetonitrile-water (50:50 w/w) showed the excellent efficiency indicated by baseline-resolved of each peak of alkyl benzenes. The resulted separation efficiency by employing gradient elution mode exceeded its counterpart (isocratic mode), which is indicated by better in peak capacity, retention factor, and number theoritical plate. Two different mobile phases for gradient elution mode, composed of A that contain of acetonitrile-water (40/60 w/w) and B that contain of acetonitrile-water (60/40 w/w) were utilized in the range of 20-40 min for 0-100% B. It was found that increasing gradient time strongly affect to the retention factor and peak capacity.<strong></strong></p>
Hepatitis B virus (HBV) still remains a major global public health problem. One-half to one-third of the total HBV infected people died due to late detection of HBV. Serological antigen and viral HBV detections can help in the diagnosis, referral, and treatment of HBV. Available methods for HBV detection mostly used bulky instruments. Miniaturization of devices for HBV detection has been started by narrowing down the size of the devices. Several methods have also been proposed to increase the selectivity and sensitivity of the miniaturized methods, such as sandwich recognition of the biomarkers and the use of nano- to micro-sized materials. This review presents recent HBV detections in the last two decades from laboratory-based instruments towards microfluidic paper-based analytical devices (µPADs) for point-of-care testing (POCT) purposes. Early and routine analysis to detect HBV as early as possible could be achieved by POCT, especially for areas with limited access to a central laboratory and/or medical facilities.
Nanobiocatalyst microreactor column of an immobilized trypsin onto nanoporous monolithic polymer has been developed. In this work, a poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EDMA)) monolih with total monomer %T 40 and cross-linker %C 25 was prepared as a nanoporous polymeric-support by in-situ copolymerization inside the microbore silicosteel tubing (1.0 mm i.d x 100 mm). This nanoporous monolithic-support was chemically modified with trypsin through the ring-opening reaction of epoxide group to form a nanobiocatalyst microreactor. In order to obtain reliable microreactor, some parameters such as immobilization time (amount of trypsin), and effect of glutaraldehyde activation on the trypsin-immobilized monolithic column were optimized. The pore size distribution, morphology and elemental composition of the monolith were studied by inverse size exclusion chromatography (ISEC) and scanning electron microscopy (SEM-EDX), respectively. While permeability of the monolith was obtained through measurement of its back pressure using HPLC pump. The monolith was predominated by mesoporous content (65.85%) while the flow-through pore was 28.20%. From SEM-EDX, it was found that the highest nitrogen content (12.40%), which indicates the amount of trypsin chemically attached on the monolitic support, was found in the nanobiocatalyst microreactor with immobilization time 4h without glutaraldehyde as activation agent. The optimized nanobiocatalyst microreactor column was successfully applied to a rapid and efficient digestion of protein samples in the range of second to minute.Keywords: nanoporous monolit, immobilized enzyme, nanobiocatalyst microreactor, protein digestion Pada penelitian ini telah dilakukan pengembangan pembuatan kolom nanobiokatalis mikroreaktor yang terimobilisasi oleh enzim tripsin pada polimer monolitik nanopori. Polimer monolitik nanopori dibuat secara insitu kopolimerisasi dalam kolom silicosteel (1.0 mm i.d x 100 mm) dari monomer GMA dan EDMA sebagai crosslinker serta penambahan porogen dengan komposisi 1-propanol/1,4-butanediol/air (7:4:1) dan inisiator radikal AIBN 1%. Proses polimerisasi berlangsung selama 12 jam pada suhu 60°C dengan persentase total monomer %T 40 dan persentase crosslinker %C 25. Monolit dimodifikasi secara kimia menggunakan metode imobilisasi enzim melalui reaksi pembukaan cincin *Penulis korespondensi
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