Abstract. We investigate the potential for aircraft-based top-down emission rate retrieval over- and under-estimation using a regional chemical transport model, the Global Environmental Multiscale-Modeling Air-Quality and CHemistry (GEM-MACH). In our investigations we consider the application of the mass-balance approach in the Top-down Emission Rate Retrieval Algorithm (TERRA). Aircraft-based mass-balance retrieval methodologies such as TERRA require relatively constant meteorological conditions and source emission rates to reliably estimate emission rates from aircraft observations. Avoiding cases where meteorology and emission rates change significantly is one means of reducing emissions retrieval uncertainty, and quantitative metrics that may be used for retrieval accuracy estimation are therefore desirable. Using these metrics has the potential to greatly improve emission rate retrieval accuracy. Here, we investigate the impact of meteorological variability on mass-balance emission rate retrieval accuracy by using model-simulated fields as a proxy for real-world chemical and meteorological fields, in which virtual aircraft sampling of the GEM-MACH output was used for top-down mass balance estimates. We also explore the impact of upwind emissions from nearby sources on the accuracy of the retrieved emission rates. This approach allows the state of the atmosphere used for top-down estimates to be characterized in time and 3D space; the input meteorology and emissions are “known”, and thus potential means for improving emission rate retrievals and determining the factors affecting retrieval accuracy may be investigated. We found that emissions retrieval accuracy is correlated with three key quantitative criteria, evaluated a priori from forecasts and/or from observations during the sampling period: (1) changes to the atmospheric stability (described as the change in gradient Richardson number), (2) variations in the direction of transport, as a result of plume vertical motion and in the presence of vertical wind shear, and (3) the combined effect of the upwind-to-downwind concentration ratio and the upwind-to-downwind concentration standard deviations. We show here that cases where these criteria indicate high temporal variability and/or high upwind emissions can result in “storage-and-release” events within the sampled region (control volume), which decrease emission rate retrieval accuracy. Storage-and-release events may contribute the bulk of mass-balance emission rate retrieval under- and over-estimates, ranging in the tests carried out here from −25 % to 24 % of the known (input) emissions, with a median of −2 %. Our analysis also includes two cases with unsuitable meteorological conditions and/or significant upwind emissions to demonstrate conditions which may result in severe storage, which in turn cause emission rate under-estimates by the mass-balance approach. We also introduce a sampling strategy whereby the emission rate retrieval under- and over-estimates associated with storage-and-release are greatly reduced (to −14 % to +5 %, respectively, relative to the magnitude of the known emissions). We recommend repeat flights over a given facility and/or time-consecutive upwind and downwind (remote) vertical profiling of relevant fields (e.g., tracer concentrations) in order to measure and account for the factors associated with storage-and-release events, estimate the temporal trends in the evolution of the system during the flight/sampling time, and partially correct for the effects of meteorological variability and upwind emissions.
Many signaling pathways act through shared components, where different ligand molecules bind the same receptors or activate overlapping sets of response regulators downstream. Nevertheless, different ligands acting through cross-wired pathways often lead to different outcomes in terms of the target cell behavior and function. Although a number of mechanisms have been proposed, it still largely remains unclear how cells can reliably discriminate different molecular ligands under such circumstances. Here we show that signaling via ligand-induced receptor dimerization-a very common motif in cellular signaling-naturally incorporates a mechanism for the discrimination of ligands acting through the same receptor.
The Type I Interferon family of cytokines all act through the same cell surface receptor and induce phosphorylation of the same subset of response regulators of the STAT family. Despite their shared receptor, different Type I Interferons have different functions during immune response to infection. In particular, they differ in the potency of their induced anti-viral and anti-proliferative responses in target cells. It remains not fully understood how these functional differences can arise in a ligand-specific manner both at the level of STAT phosphorylation and the downstream function. We use a minimal computational model of Type I Interferon signaling, focusing on Interferon-α and Interferon-β. We validate the model with quantitative experimental data to identify the key determinants of specificity and functional plasticity in Type I Interferon signaling. We investigate different mechanisms of signal discrimination, and how multiple system components such as binding affinity, receptor expression levels and their variability, receptor internalization, short-term negative feedback by SOCS1 protein, and differential receptor expression play together to ensure ligand specificity on the level of STAT phosphorylation. Based on these results, we propose phenomenological functional mappings from STAT activation to downstream anti-viral and anti-proliferative activity to investigate differential signal processing steps downstream of STAT phosphorylation. We find that the negative feedback by the protein USP18, which enhances differences in signaling between Interferons via ligand-dependent refractoriness, can give rise to functional plasticity in Interferon-α and Interferon-β signaling, and explore other factors that control functional plasticity. Beyond Type I Interferon signaling, our results have a broad applicability to questions of signaling specificity and functional plasticity in signaling systems with multiple ligands acting through a bottleneck of a small number of shared receptors.
Abstract. We investigate the potential for aircraft-based top-down emission rate retrieval over- and under-estimation using a regional chemical transport model, the Global Environmental Multiscale-Modeling Air-Quality and CHemistry (GEM-MACH). In our investigations we consider the application of the mass-balance approach in the Top-down Emission Rate Retrieval Algorithm (TERRA). Aircraft-based mass-balance retrieval methodologies such as TERRA require relatively constant meteorological conditions and source emission rates to reliably estimate emission rates from aircraft observations. Avoiding cases where meteorology and emission rates change significantly is one means of reducing emissions retrieval uncertainty, and quantitative metrics that may be used for retrieval accuracy estimation are therefore desirable. Using these metrics has the potential to greatly improve emission rate retrieval accuracy. Here, we investigate the impact of meteorological variability on mass-balance emission rate retrieval accuracy by using model simulated fields as a proxy for real world chemical and meteorological fields, in which virtual aircraft sampling of the GEM-MACH output was used for top-down mass balance estimates. We also explore the impact of upwind emissions from nearby sources on the accuracy of the retrieved emission rates. This approach allows the state of the atmosphere used for top-down estimates to be characterized in time and 3D space; the input meteorology and emissions are “known”, and thus potential means for improving emission rate retrievals and determining the factors affecting retrieval accuracy may be investigated. We found that emissions retrieval accuracy is correlated with three key quantitative criteria, evaluated a priori from forecasts and/or from observations during the sampling period: (1) changes to the atmospheric stability (described as the change in gradient Richardson number), (2) variations in the direction of transport, as a result of plume vertical motion and in the presence of vertical wind shear, and (3) the combined effect of the upwind to downwind concentration ratio and the upwind to downwind concentration standard deviations. We show here that cases where these criteria indicate high temporal variability and/or high upwind emissions can result in “Storage-and-Release” events within the sampled region (control volume), which decrease emission rate retrieval accuracy. Storage-and-release events may contribute the bulk of mass-balance emission rate retrieval under- and over-estimates, ranging in the tests carried out here from −25 % to 24 % of the known (input) emissions, with a median of −2 %. Our analysis also includes two cases with unsuitable meteorological conditions and/or significant upwind emissions to demonstrate conditions which may result in severe storage, which in turn cause emission rate underestimates by the mass-balance approach. We also introduce a flight strategy whereby the emission rate retrieval under- and over-estimates associated with storage-and-release are greatly reduced (to −14 % to +5 % respectively, relative to the magnitude of the known emissions). We recommend repeat flights over a given facility in order to measure and account for the factors associated with storage-and-release events – to estimate the temporal trends in the evolution of the system during the flight/sampling time, and partially correct for the effects of meteorological variability and upwind emissions. Our analysis allows the increased costs associated with longer flight times to be weighed against quantitatively reduced uncertainty in the emission rate estimates.
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