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Macroalgae produce a wide variety of chemically active secondary metabolites. Brown seaweed; Sargassum vulgare and its associated seaweeds were collected from Hurghada Red sea coast of Egypt. Optimal physicochemical properties; slightly alkaline; low turbidity, moderate temperature and available nutrient content of saline water produced massive growth of S. vulgare (53% covering percentage) during autumn (2018). Heavy metals accumulation inside the investigated seaweed S. vulgare was within the usually range. The associated macroalgal species with S. vulgare are belonging to 9 genuses, 12 species. Chromatography, mass spectrophotometry (GC/MS) analysis revealed the presence of Twenty five bioactive compounds. The major phytochemical constituents in the chloroform extract of S. vulgare are n-Hexadecanoic acid 28.29 %, Heptacosane 8.04 %, trans-13-Octadecenoic acid 5.50 %, Oleic Acid 4.24 %, Palmitoleic acid 3.56 % and Hexadecanoic acid, ethyl ester 3.30 %.

Anabaena variabilis was collected by isolation from saline soil at Elhamol city, Kafr Elsheikh governerate, Egypt. Spirulina platensis was obtained from cyanobacteria Lab., Microbiology Dept., Sakha Agricultural Research Station, Egypt. GC/MS analysis showed that 37 and 7 bioactive compounds from A. variabilis and S. platensis were identified, respectively. Moreover, the area percentage of the same bioactive compound varied according to the type of cyanobacteria. The major phytochemical constituents in the methanol extract of A. variabilis are Pentadecanoic acid 29%, 12,15-Octadecadienoic acid methyl ester 10.26%, Hexadecenoic acid methyl ester (Z) 8.3%, Thieno [3,4-C] pyridine,1,3,4,7-tetraphenyl 5.86%. While, S. platensis methanol extract contain Hexadecanoic acid 29%, 9,12,15-Octadecadienoic acid methyl ester 24% and 9,12 Octadecatrienoic acid zz methyl ester, 24.36%.

ARGASSUM latifolium is an edible brown alga collected from the red seashore in Egypt during autumn. This research aimed to investigate the physicochemical properties and biogenic molecules of S. latifolium. The optimal physicochemical properties of red seawater were a moderate temperature (24°C), salinity (40.12%), hydrogen ion concentration (7.8), dissolved oxygen (7.37mg/L), a slightly alkaline pH, a slightly elevated biochemical oxygen demand (1.8mg/L), and an electrical conductivity of 53.67mmhos/cm. S. latifolium's biochemical composition is rich in carbohydrates (45.52mg/g dry weight) and low in protein (6.38mg/g dry weight). S. latifolium contained 89.23% and 23.37% moisture and ash, respectively. The bioactive compounds in an aqueous crude extract of S. latifolium separated between 200 and 400nm, as shown by UV scan analyses. The FT-IR analysis of S. latifolium revealed numerous functional groups, including -OH, -NH, -CH, -COOH, CO, and C-C. The resulting aqueous extract of S. latifolium contained steroids, terpenoids, flavonoids, phenols, coumarins, and quinones.