Isoniazid, the choice antitubercular agent, has only been employed against Mycobacterium tuberculosis. This study evaluated if the enzyme-mimetic activities of magnetic nanoparticles could accelerate the activation process of isoniazid against mycobacterial and, more importantly, non-mycobacterial microorganisms. First, magnetic nanoparticles were synthesized and coated by lipoamino acid; then, isoniazid was conjugated to synthesized nanoparticles. Antibacterial activities of nanoconjugated isoniazid were evaluated against Mycobacterium tuberculosis and four Gram-positive and Gram-negative nonmycobacterial strains through a microdilution broth process. Results showed that the required amount of isoniazid against Mycobacterium tuberculosis would decrease to 44.8% and 16.7% in conjugation with naked and surface-modified magnetic nanoparticles, respectively. Also, 32 μg/mL and 38 μg/mL of isoniazid in conjugation with naked and surface-modified nanoparticles, respectively, could prevent the growth of Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Hence, the vicinity of magnetic nanoparticles with isoniazid could declare promising aspects of isoniazid antibacterial capabilities.
Magnetic cell immobilization has been introduced as a novel, facile and
highly efficient approach for cell separation. A stable attachment between bacterial cell wall with
superparamagnetic iron oxide nanoparticles (SPIONs) would enable the microorganisms to be affected
by an outer magnetic field. At high concentrations, SPIONs produce reactive oxygen species
in cytoplasm, which induce apoptosis or necrosis in microorganisms. Choosing a proper surface
coating could cover the defects and increase the efficiency.
In this study, asparagine, APTES, lipo-amino acid and PEG surface modified SPIONs
was synthesized by co-precipitation method and characterized by FTIR, TEM, VSM, XRD, DLS
techniques. Then, their protective effects against four Gram-positive and Gram-negative bacterial
strains including Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Pseudomonas
aeruginosa were examined through microdilution broth and compared to naked SPION.
The evaluation of characterization results showed that functionalization of magnetic nanoparticles
could change their MS value, size and surface charges. Also, the microbial analysis revealed
that lipo-amino acid coated magnetic nanoparticles has the least adverse effect on microbial
strain among tested SPIONs.
This study showed lipo-amino acid could be considered as the most protective and
even promotive surface coating, which is explained by its optimizing effect on cell penetration and
negligible reductive effects on magnetic properties of SPIONs. lipo-amino acid coated magnetic
nanoparticles could be used in microbial biotechnology and industrial microbiology.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.