Six simple and sensitive spectroscopic and conductometric procedures (A-F) were developed for the determination of tramadol hydrochloride. Methods A, B and C are based on the reaction of cobalt (II) thiocyanate with tramadol to form a stable ternary complex, which could be measured by spectrophotometric (method A), atomic absorption (method B) or conductometric (method C) procedures. Methods D and E depend on the reaction of molybdenum thiocyanate with tramadol to form a stable ternary complex, measured by spectrophotometric means (method D) or by atomic absorption procedures (method E), while method F depends on the formation of an ion pair complex between the studied drug and bromothymol blue which is extractable into methylene chloride. Tramadol hydrochloride could be assayed in the range of 80-560 and 40--220 μg ml -1 , 1-15 mg ml -1 and 2.5-22.5, 1.25-11.25 and 5-22 μg ml -1 using methods A,B,C,D,E and F, respectively. Various experimental conditions were studied. The results obtained showed good recoveries. The proposed procedures were applied successfully to the analysis of tramadol in its pharmaceutical preparations and the results were favorably comparable with the official method.
Four simple, sensitive and reproducible methods were developed for the determination of gabapentin (GPT) in pure form and in pharmaceutical preparations. Methods A and B are based on the reaction of cupric chloride with gabapentin to form stable complex, which could be measured spectrophotometrically at λmax246 nm (method A) or by using conductometric technique (method B). While method C and D depends on the formation of ion pair complex between the studied drug and bromothymol blue, bromocresol green respectively this was extractable with methylene chloride. The concentration ranges were 40-95 μg mL-1, 1-15 mg, 100-800 and 10-150 μg mL-1for methods A, B, C and D respectively .The optimization of various experimental conditions were described .The results obtained showed good recoveries, Ringbom optimum concentration ranges were calculated, in addition to molar absorptivity and sandell’s sensitivity, detection and quantification limits. The methods were successfully applied to the determination of GPT in bulk and pharmaceutical preparations. The results were favorably comparable with the official method. The molar combining ratio for methods (A-B) was found to be (2:1) (drug: reagent) while for method (C-D) it was found to be (1:1).
All reagents and solvents were of analytical grade, doubly distilled water was used. • Disodium hydrogen phosphate (El-Nasr Chemical Co. Cairo, Egypt), 0.05 M aqueous solution. • 4-chloro-7-nitrobenzofurazan (NBD-Cl) (Fluka-Germany), a fresh solution (0.1% w/v) in methanol was prepared daily. • Betahistine dihydrochloride pure drug (Pharco Co., Egypt),
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