In the present study, the chromosomal analysis of mulberry silkworm, Bombyx mori L. was carried out in order to study its chromosome behaviour and karyotype from larval gonadal tissue. The observed diploid number was 2n=56. The mean total length, RL% and TCL% were measured at metaphase stage of gametogenesis. The chromosomal behaviour during meiotic stages viz., leptotene, zygotene, pachytene, diplotene, diakinesis and anaphase were also analyzed. Results indicate that the samples studied were males with a diploid chromosome number of 2n=56. …………………………………………………………………………………………………….... Introduction:-The order Lepidoptera includes moths and butterflies. It is a species rich order and among this order are some commercially important insects like silkmoths and many serious agricultural pests too.The mulberry silkworm is a commercially important insect which is completely domesticated and one of the most genetically studied insect and well known as lepidopteran model insect (Nagaraju and Goldsmith, 2002) apart from its importance as a producer of silk-"the queen of textiles".As in most Lepidoptera, the chromosomes of Bombyx mori L. are holocentric (Murakami and Imai, 1974) i.e. they possess centromeres throughout the chromosome body. These chromosomes are highly condensed and appear dotshaped at mitotic and meiotic metaphase stages. Their diffused centromeres and lack of special features make them difficult to identify individually. The chromosome number is known with n=28 (Kawaguchi,1928) and 2n=56 (Kawamura 1979, Yoshido et al 2005. In the present investigation, an attempt has been made to prepare a karyotype of Bombyx mori L. on the basis of decreasing chromosome length from meiotic metaphase complement. The chromosome behavior during the meiotic stages was also studied from the larval gonadal cells. Material and Methods:-Rearing of silkworm larvae:-The IIIrd instar larvae of mulberry silkworm, B.mori L. were collected from RSRS Miransahib, Jammu and transported to Cytogenetics Lab., Dept. of Zoology., University of Jammu. About 20 larvae were reared in rearing cage for further development ( fig. 1). The temperature and relative humidity was maintained as 26˚C and 80% RH for the IIIrd instar, 24-25˚C and 75% RH for IV instar and 23-24˚C temperature and around 70% RH for the V instar, the larvae were fed with mulberry leaves during larval period.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.