Reproductive senescence is accompanied by a reduced number and quality of ovarian follicles in response to the accumulation of free radicals and the process of apoptosis. Having selected mice as models, we examined the hypothesis that curcumin as an antioxidant and anti-inflammatory agent might prevent or retard ovarian aging. Female NMRI 21-day-old mice were divided into control, vehicle and curcumin groups. In the treatment group the mice received curcumin at 100mgkg–1day–1 intraperitoneally. After 6, 12 and 33 weeks several parameters were examined including ovarian reserve, oocyte quality, oxidative status, invitro fertilisation and expression of ovulation-related (growth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15)) and anti-aging-related (sirtuin 1 (SIRT-1) and SIRT-3) genes. Curcumin treatment up to 12 and 33 weeks resulted in increased ovarian volume and number of follicles and was associated with elevated anti-Müllerian hormone and oestrogen and diminished FSH serum levels. Furthermore, enhanced oocyte maturation, fertilisation and embryo development plus reduced oxidative stress were seen in the curcumin group. Also, the expression of GDF-9, BMP-15, SIRT-1 and SIRT-3 genes was increased in the curcumin group. Concerning gestational age, the findings of the study suggested that administration of curcumin could delay the process of oocyte aging in a mouse model.
Objectives: Oxidative stress can initiate the process of apoptosis which affects the oocyte quality and reduces development competency in the ovarian follicles. Accordingly, the present study determined the effects of curcumin as a well-known antioxidant on the apoptosis prevention of mature oocytes during the natural increasing age in female mice. Materials and Methods: In this case-control, interventional, and quantitative applied research, 21-day-old NMRI (Naval Medical Research Institute) female mice were used as control, vehicle, and curcumin groups. The mice in the curcumin group received 100 mg/kg/d curcumin intraperitoneally. After initial interventions, the Annexin-V-FLUOS staining was applied to evaluate the oocyte apoptosis rate in the three groups at 6, 12, and 33 weeks of age. The expression of oocytes apoptosis-related genes (Bcl2 and Bax) was also assessed by the real-time polymerase chain reaction (PCR) technique, followed by measuring oxidation-reduction markers in the ovaries. Results: Our results showed that oocyte apoptosis and necrosis in the curcumin group decreased in comparison with the control and vehicle groups at 12 and 33 weeks (P<0.001). Moreover, the use of curcumin led to the upregulation of Bcl2 and downregulation of Bax genes at 6, 12, and 33 weeks (P<0.001). In addition, the superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities increased in the curcumin group compared with control and vehicle groups at 12 and 33 weeks (P<0.001) while malondialdehyde (MDA) decreased in the curcumin group at 12 (P<0.001), in the control at 12 (P<0.01), and in the vehicle at 33 weeks (P<0.01). Conclusions: In general, curcumin could suppress oocyte apoptosis through upregulating Bcl2 and the downregulating of Bax gene, as well as suppressing oxidative stress pathways involving oocyte apoptosis and necrosis.
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