An indigenous Bacillus thuringiensis strain B.t.LDC-391 producing cytocidal proteins against human colon cancer cell line, HCT-116,
was subjected to phenotypic and genotypic characterization to evaluate its relatedness to B.anthracis. The morphological features of
this strain were meta-analyzed with data of other parasporin and insecticidal protein producing Bacillus thuringiensis strains. The
conventional biochemical analysis and antibiotic sensitivity test proved it as an ampicillin resistant which is a salient feature,
absent in B.anthracis Ames. PCR analysis showed the absence of cyt and parasporin related genes in the genome of B.t.LDC-391. But
the strain was positive for cap gene. The sequencing and bio-informatic analysis of cap gene and 16S rDNA of B.t.LDC-391 placed
it closer to B.thuringiensis and revealed significant divergence from that of any B.anthracis strain. However our strain lacked β–
hemolysis on human erythrocytes which is a common feature of B.anthracis strains and parasporin producers.
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