Background: Medicinal plants and green synthesis of silver nanoparticles (AgNPs) have proven to be good sources of agents effective in the treatment of diabetes mellitus. The present study focused on the green synthesis of AgNPs from the aqueous leaf extract of Pouteria sapota in order to evaluate the in vitro and in vivo antidiabetic properties of this extract and the synthesized AgNPs. Methods: The AgNPs were biologically synthesized under ambient conditions from an aqueous leaf extract of P. sapota using the hot percolation method and were characterized using spectroscopic methods, X-ray diffraction, and scanning electron microscopy. The in vitro antidiabetic activity of the aqueous leaf extract and AgNPs was confirmed by non-enzymatic glycosylation of hemoglobin, glucose uptake by yeast cells following exposure of cells to 5 or 10 mmol/L glucose solution, and inhibition of α-amylase. Further, in vivo antidiabetic activity was assessed in streptozotocin-induced rats. Rats were treated with aqueous leaf extract (100 mg/kg) or AgNPs (10 mg/kg) for 28 days. Following treatment, rats were killed for biochemical and histopathological analysis of kidney and liver samples. Results: A significant reduction in blood sugar levels was noted in rats treated with leaf extract or AgNPs. Results of in vitro and in vivo analyses in rats treated with leaf extract or AgNPs show that both the extract and the biologically synthesized AgNPs have antidiabetic activity. Conclusion: The aqueous leaf extract of P. sapota and AgNPs exhibited efficient antidiabetic activity in the rat model of diabetes and therefore could have potential for development for medical applications in the future.
Medicinal plants have been used as therapeutic drug throughout the world. But, a very few of them have been scientifically validated. Samanea Saman belongs to the Leguminosae family commonly known as rain tree and is native to tropical America. S.saman tree has different parts like a leaf, fruits, pods, seeds, and wood. S.saman is currently studied for the production of biogas which is easily obtained in the urban and rural areas. S.saman has been used in traditional medicine as a remedy for the treatment of different diseases. The phytochemical screening of the plant revealed the presence of alkaloids-C 8 H 17 ON and C 17 H 36 ON 3 pithecolobine and saponin (samarin). Natural products are mainly derived from medicinal plants, which are tested in vitro and in vivo models and used to investigate the mechanism of action of drugs with potential biological properties. Additionally, S.saman were recommended as the suitable tree for planting in the urban environment as this species can reduce the outdoor temperature for all types of ground covers. This review is mainly focused on antioxidant, antibacterial, anti-diabetic potential, insecticidal, antifungal, analgesic, anti-ulcer and cytotoxic activities. However, this study helps to develop new strategies for the designing of novel drugs to treat various diseases related to human race.
In recent years, diabetes and obesity have become a major problem in global health care because of changes in lifestyle, food habits, and age‐related metabolic disorders. Diabetes mellitus is one of the most common diseases, affecting millions of people worldwide. Currently, herbal drugs are used to control obesity and diabetes. The present study investigates the anti‐obesity, antidiabetic, and antioxidant activities of Samanea saman leaf extract. A methanolic extract of
S. saman leaves was prepared by a maceration method. The
S. saman leaf extract was studied for its inhibitory effect on glucose utilization using specific in vitro procedures to analyze its antioxidant, anti‐obesity, and antidiabetic activities via different assays, such as α‐amylase and α‐glucosidase inhibition assay, glucose uptake by yeast cells, nonenzymatic glycosylation assay followed by glucose diffusion assay. The outcome of the study showed that the methanolic extract strongly inhibited the pancreatic lipase, α‐amylase, and glucosidase activities, compared with the standard drug. The results showed that the extract possessed considerable antioxidant and antidiabetic activities, and further studies are needed to confirm the results using an in vivo model. Thus, it is proposed that
S. saman can be used as a therapeutic agent.
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