is a commonly found pathogen that can cause food-spoilage and life threatening infections. However, the potential molecular effects of natural active thymol molecules and chitosan silver nanoparticles (C@AgNPs) in bacteria remain unclear. This gap in the literature has prompted us to study the effects of thymol loaded chitosan silver nanoparticles (T-C@AgNPs) against biofilm associated proteins in methicillin-resistant (Bap-MRSA) 090 and also their toxicity, anti-cancer activity, and validation of their molecular docking. The results showed excellent antibacterial activity of T-C@AgNPs against Bap-MRSA 090, having a minimum inhibitory concentration of 100 μg mL and a 10.08 ± 0.06 mm zone of inhibition (ZOI). The cyclic voltammogram (CV) analysis clearly showed pore forming of T-C@AgNPs at 300 μg mL concentration, and evidence of the interruption of the electron transport chain was clearly seen. The 200 μg mL concentration exhibited a 52.60 ± 0.25% anti-biofilm property by T-C@AgNPs against Bap-MRSA 090. The T-C@AgNPs showed no toxicity to peripheral blood mononuclear cells (PBMC) (IC = 221 ± 0.71 μg mL) compared to the control, and anti-cancer activity against human triple negative breast cancer cell line (MDA-MB-231) (IC 110 ± 1.0 μg mL) compared to the standard drug Doxorubicin (IC = 19 ± 1.0). The excellent properties of T-C@AgNPs were validated by molecular docking studies and showed best match scoring to target proteins compared to standards. These excellent properties of T-C@AgNPs highlight for the first time its pharmacology and potential in medicinal drug development applications for future research.
Oral diseases continue to be a major health problem world wide. It can be treated with antibiotics which lead to side effects so an alternatives use of natural herbal extracts. The aim of the study is to evaluate the a ntibacterial and antibiofilm activity of Vaccinium macrocarpon Aiton fruit extract. Then, we attempted to formulate the juice in the form of a polymer gel to aid topical application. V. macrocarpon (Cranberry) components possess potential anti-caries agents and, hence they inhibit acid formation, attachment and biofilm formation by microorganisms. V. macrocarpon exhibited bacteriocidal effect against oral microbes. Cranberry extract acted as a potent antibiofilm properties and prevents biofilm formation. The methanolic extract of cranberry was found to becytotoxic against the causative microorganisms. The time kill assay also confirmed the bacteriostatic action of the extract. The cranberry extract was formulated into a gel for applying to treat dental caries.
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