Alfalfa (Medicago sativa L.) was grown in hydroponic culture to investigate adaptation to Fedeficiency. Root exudates released into the nutrient solution from Fe-deficient plants were trapped and condensed on an amberlite XAD-4 resin column. The diethyl ether fraction of these exudates dissolved ferric phosphate remarkably. The dissolving capability was about 62 times higher than that of root exudates obtained from Fe-sufficient plants in complete nutrient solution. The Fe-dissolving compound was separated and identified. It was a new natural compound with molecular formula C~4H~005 and was identified as 2-(3',5'-dihydroxyphenyl)-5,6-dihydroxybenzofuran by means of mass spectrometry and 1H-nuclear magnetic resonance. This new compound worked as a phytoalexin and inhibited completely the fungal growth of Fusarium oxysporum f. sp. phaseoli.
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