Studies on the assessment of anti-nutrient and vitamin compositions of Pleurotus ostreatus cultivated on three agrowastes materials were carried out in Dilomat Farms and Services Limited and Department of Plant Science and Biotechnology, Rivers State University. Ten treatments were obtained from the mixtures of cassava bran, wood ash and sawdust at different concentrations which also comprised the control and combined effect. Vitamins assessed were Vitamins A, C, Thiamine and Niacin. The combined effect (CE) had the highest contents of these vitamins investigated. However, minimal concentrations of Vitamin C was recorded. Anti-nutrient analysis showed that the CE treatment had highest values of phytate (1.95±0.00), oxalate (0.71±0.02) and flavonoid (3.52±0.03). Although,the highest values of tannin (7.20±0.00) and polyphenol (2.50±0.00) were recorded for sawdust and cassava bran (SCB) treatment. The Highest concentration of saponin (0.08±0.00) was observed for sawdust and wood ash (SWA) treatment than others. Nevertheless, there was no fruit recorded for higher concentrations of SCB treatment due to contamination. The cyanide concentrations (0.03±0.02 to 0.52±0.01) for treatments containing cassava bran were all within the acceptable limit. Generally, the combined effect treatment performed better than every other treatment utilized.
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