Obesity is a condition in which large amount of fat is stored in adipose tissue. Obesity is the greatest risk for many diseases like coronary heart disease, type 2 diabetes millions associated with insulin resistance, arthritis disorder, hypertension and cancers. Currently, the available drugs for obesity have been associated with number of side effects when compared with allopathic drugs. Salvia hispanica was one of the member of Lamiaceae family, collected to study the antiobesity activity. Extraction of Salvia hispanica using different solvents was done and tested for the presence of phytochemical constituents and the antimicrobial activity was monitored to evaluate the zone of inhibition. Further, antioxidant potential activity such as DPPH, FRAP and H2O2 assay was studied. Percentage of inhibition of Salvia hispanica was calculated and was observed as in capturing the free radicals present in the body. The surface and cross-sectional morphology Salvia hispanica extract nanoparticles was examined by using SEM. High ALA content make chia a perfect as it is associated with lower incidence of Cardiovascular diseases. This metabolic syndrome is mediated by inflammatory pathways. Hence, the in vitro activity of anti-inflammatory assay was performed by inhibition of albumin denaturation and anti-obesity activity was performed by lipase inhibition assay. Thus the result indicates that the seed extracts of Salvia hispanica possess antiobesity activity.
Keywords: Salvia hispanica, anti-obesity, cholesterol, Antioxidant, lipase inhibition
Objective: The current study is to evaluate the antimicrobial, antioxidant, anti-inflammatory, and in vitro cytotoxicity activities of polyhydroxybutyrate (PHB) and to develop the herbal impregnated PHB cast film for wound healing activities using Albino Wistar rat model.
Methods: PHB produced by Azotobacter chroococcum A3 strain was synthesized and characterized (previous study). The PHB was subjected to various biocompatibility studies such as antimicrobial, antioxidant, and anti-inflammatory studies. The PHB was also subjected to cytotoxicity study by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. PHB films were made using different combinations of plant and algal blends (herbal blends). The herbal blends of PHB films were evaluated for in vivo wound healing activity using Albino Wistar rats.
Results: The turmeric impregnated PHB showed the highest result for antimicrobial with 27.25±0.23 mm against skin pathogens and antioxidant activity with the highest percentage of inhibition of 76%. The result predicts that PHB will not let to any toxic substances rather it acts as a chemoprotective agent followed by the inhibitory concentration value was found to be 1.56 μg/ml for 100 μg. The in vivo study showed better wound healing activity for PHB blended with 2% turmeric leaf and rhizome cast film. Whereas the wound healing activity of control and crude PHB was 90.4±0.4 and 91.3±0.56 respectively.
Conclusion: The results from the present study showed that PHB can act as a good candidate for drug carrier and it is biocompatible in living cells.
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