Glabrata to azole derivates, widely used since the 1980s [5,6]. Currently, despite increasing clinical concern, the epidemiology of C. Glabrata remains poorly known compared to that of C. Albicans. While C. Glabrata is considered a commensal of the human digestive tract, its natural reservoir is still uncertain. The use of molecular technique to determine genotype of clinical isolates can provide useful clues for estimating the reproductive mode, geographic prevalence's, and cross transmission of bloodstream infections between patients [7-12]. Possible transmission between patients has been suggested and clusters of invasive infections have been reported [13-15]. Molecular typing method would allow better understanding of the emergence of this species, notably in a hospital context. In the present study, we aimed to analyze by polymorphic markers a collection of C. Glabrata isolates from various sites in hospitalized and non hospitalized patients to look for association of variability with patient population. Materials and Methods Patients and isolates A collection of C. Glabrata clinical isolates were collected in a prospective study between January 2005 and December 2011. The isolates were subdivided into outpatients and inpatients clinic populations of Habib Bourguiba Sfax-Hospital (Table 1). The first population included 108 isolates from in-patients and were subdivided as described in Table 1. Fifty nine invasive isolates from blood culture collected from different wards (intensive care unit, infectious disease unit, nephrology or endocrinology unit) of which 15 isolates were previously tested [3], and 49 isolates from urine samples. The second population contained 24 vaginal isolates and thirteen buccal isolates taken from oral cavities. All were collected from the outpatients at the same period [16]. All isolates were selected as only one isolate was considered for each patient and we were certain that patients from the outside hospital had never presented a positive culture from any site for C. glabrata and hadn't been hospitalized in the last two years.
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