This study was aimed to identify and confirm of E.coli isolated from arthritis in chickens. Samples for the isolation of the bacteria were taken from broiler chickens with arthritis symptoms (swelling hock joint), and then examined by culturing, VITEK test as well as molecular assay. Antimicrobial susceptibility of bacterial isolate was done. The results showed pink colour colonies on MacConkey agar. The VITEK system was used to identify those colonies. The results revealed that the isolate gave 97 % parallel to those features of E. coli equally recognized by the standard gram negative card. The isolate was found to be sensitive to ticarcillin/clavulanic acid and many others antibiotics and resistance to ticarcillin as well as for other types of antibiotics .The results of the 16Sr RNA gene revealed that E.coli primers of the16S rRNA gene had successfully targeted the respective gene and have shown the single bands of the16S RNA gene at 1500 bp. Sequencing of this gene was performed for the isolate. The results of nucleotide sequencing were submitted in Gene bank database and have accession number: ID: MT012194.1. The phylogenetic analysis Of the isolate was 100% similar to USA:CP048605, Pakistan:GU594300, VitNamHatey: AP022650 and China:MN208222, however, it was 99% similar to India:LCO58573.
The fermentable fiber, Vitacel R200 is industrial compound cellulose exerting many beneficial health effects at the hosts. In this study, rainbow trout (Oncorhynchus mykiss) fingerling (120 ± 4.5 grams) were fed dietary 1% (10 g/kg feed) Vitacel R200 for 7 weeks, then challenged with Aeromonas hydrophila. Two weeks after the challenge, hemato-biochemical and immune parameters in the challenged fish were studied. The lysozyme and complement activity, as well as, white blood cells (WBCs), hemoglobin (Hb%), percentage of neutrophil and eosinophil in the fish fed Vitacel were significantly increased compared with the control group. Furthermore, the serum total protein, albumin, Na+, K+, P, and Ca++ concentrations in the fish fed diet containing 1% Vitacel were significantly increased, while alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST), as well as, cortisol, cholesterol, and glucose concentration were significantly decreased in comparison to the control group. These results concluded that dietary Vitacel could improve the hemato-biochemical and immune parameters, as well as, the stress response in O. mykiss infected with A. hydrophila.
The present study carried out to comparative between usage of killed vaccine of Escherichia coli with aromatic oils or alone against infection of broiler with pathogenic E.coli for this purpose two hundred broiler chicks were collected and randomly divided into four groups. Each group contained fifty chicks, food and drink for all groups ad libitium. The experimental groups were treated as follows: G1 vaccinated with the killed E. coli vaccine through subcutaneous injection dose 0.5 ml (3×107 CFU/ml )in day five old and received Digestarom® P.E.P sol. in drinking water 6 ml/100L from one day old until end of trial. G2 vaccinated with killed E. coli only through subcutaneous injection dose 0.5 ml (3×107 CFU/ml) in day five old. G3 received Digestarom® P.E.P sol. in drinking water 6 ml/100Lonly one day old until end of trial. G4 control negative. The experimental birds in day 25 were challenged intratracheally inculated with Escherichia coli of 0.5 ml was LD50(3 ×108 CFU/ml) in the experiment. Blood samples were taken at day 20 and 35 for assessment of different parameters, mortality and lesions score were determined . The results showed that the use of aromatic oils and received killed vaccine of E.coli has a good effect on the immune response , decreasing the mortality rate and lesion score of E. coli infected chickens. It can be concluded from the current study that the use digestarom enhancement the immune response after vaccination with E.coli vaccine against pathogenic E.coliIt also the use E.coli vaccine with the digestarom in broiler chickens is more effective than using the vaccine alone
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