Abstract-: Early cancer diagnosis using MRI imaging is of high global interest as a noninvasive and powerful modality in molecular Imaging. Therefore demand for new MRI contrast agents, with an enhanced sensitivity and advanced functionalities that improve the targeting to specific tissues or organs, is very high. in thise study, D-glucose amine was conjugated to a well-known chelator, diethylenetriamine penta-acetic acid (DTPA), then labeled with Gd to achieve Gd-DTPA-DG, Which is a metabolic contrast agent in mMRI. The contrast agent was synthesized and characterized physicochemical using different techniques including dynamic light scattering (DLS), high resolution transmission electron microscopy (HTEM) and inductively coupled plasma atomic emission spectroscopy (ICP-AES). Efficacy of the targeted contrast agent was assessed by measuring relaxation rate in vitro and tumor MR imaging were performed to determine signal intensity (SI) in vivo ( 0.1 mmol Gd/kg ) in female balb/c mice model.According to the results, the nano metabolic contrast agent penetrate into cells and accumulated in tumor, which cause improve the contrast of tumor tissue in comparison with magnevist. The results showed that the novel nano contrast agent could become useful tool in early detection of cancer.
Abstract-Difficulties in the use, preparation, and cost of radioactively-labeled glycosylated compounds led us to this research and development study of a new gadolinium-labeled glucose compounds that do not have a radioactive half-life or difficulties in its synthesis and utilization. Despite its good resolution, Magnetic Resonance Imaging (MRI) has low sensitivity, therefor, using MRI contrast agents, such as GD-DTPA (Magnevist) will improve tissue discrimination in MRI images.The purpose of this study is the synthesis and physicochemical characterization of glycosylated gadolinium as metabolic contrast agent for molecular MRI (mMRI). In-vitro T1 relaxivity measurement and signal intensity of the glycosylated compounds has been also performed in comparison with magnevist ( GD-DTPA).Based on the structure of the 2-fluoro-2-deoxy-D-glucose molecule (FDG), first compound consisting of D-glucose conjugated to a well-known chelator, diethylenetriamine pentaacetic acid (DTPA), was syntheside , labeled with Gd to achieve Gd-DTPA-DG, another comound consisting of gadolinium oxide-based nanoparticle coating of diethyleneglycol conjugated with D-glucose via N,N-carbonyldiimidazole (CDI) mediate reaction, to achieve Gd-DEG-DG, and characterized by various analytical technique, utilizes dynamic light scattering (DLS) to determine the size distribution. The nanoparticle size and morphology were using high resolution transmission electron microscopy (HTEM). In our study, the Gd-DTPA-DG were well defined nanoparticle with size 40 nm in diameter TEM images. While Gd-DEG-DG were 10 nm . The mean hydrodynamic diameter of nanoparticles, as measured by DLS, were 300 nm and 70 nm for GD-DTPA-DG and GD-DEG-DG, respectively.The synthesized GD-DTPA-DG and GD-DEG-DG were shown higher relaxometery rates in vitro relative to magnevist. GD-DEG-DG and GD-DTPA-DG demonstrated shorter T1 than GD-DTPA at the same conectration.
Abstract-Magnetic resonance imaging (MRI) is widely used for imaging purposes. However, the sensitivity and intrinsic contrast of the MRI is low. In order to improve the quality of images Gd-DTPA (commercial Magnevist) is normally. Because of some limitations of low molecular weight of gadolinium chelates, nanoparticles gadolinium based contrast agents are proposed. In this study, we synthesized Gd-DTPA and compared its effects with Gd 2 O 3 -DEG nanoparticles. The samples were prepared at concentrations of 0.3, 0.6, 0.9 and 1.2 mM respectively by adding 1.5 ml deionized water. The corresponding Gd 2 O 3 -DEG nanoparticles diameter was measured about 80 nm. An in vitro study was performed using a 1.5 T scanner with standard spin echo protocol. Clearly, the signal amplitudes in both cases were increased with the Gd concentration at constant relaxation time. Also, a linear relation between signal intensity and longitudinal relaxation rate (R1) was observed with a correlation coefficient close to 1. The values of 4.30 and 14.27 (s -1 .mM -1 ) were achieved for Gd-DTPA and Gd 2 O 3 -DEG nanoparticles special relaxivity, respectively.
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