Nanoparticles are gaining interest in biomedical applications due to its importance such as anti-bacterial, anti-fungal and anti-cancer agents. Conventional methods for the synthesis of metal nanoparticles involves toxic reagents which produce harmful by-products and are hazardous to the environment. To overcome these limitations, green synthesis of nanoparticles was established. Eco-friendly methods using plant extracts are gaining popularity due to the abundance of raw materials and the production of non-toxic by-products threatening to the environment. Moreover, the nanoparticles synthesized from the plant extract are cost-effective. In addition, nanoparticles produced by green synthesis methods produces synergetic effect where both the nanoparticles as well as the natural bioactive constituents of the plant influence the biocidal properties. Different methods namely heating in water bath, microwave oven and exposure to bright sunlight were adopted for the synthesis of silver nanoparticles. Plant extract based synthesis of silver nanoparticles was eco-friendly and shows an alternative promise in bio-medical applications and it undertakes the negative effects of synthetic drugs.
Objective: The present study attempts to estimate the total flavonoid and phenolic content and to evaluate the free radical scavenging activity of the roots of Glycyrrhiza glabra Leguminosae family.Methods: The various extracts, namely, petroleum ether, chloroform, ethyl acetate, aqueous, and methanol of G. glabra root were evaluated by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radicals, superoxide, 2,2’-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), and non-radicals such as hydrogen peroxide (H2O2) and nitric oxide (NO) and assays. Total flavonoid and phenol contents were estimated as per the standard procedure.Results: The effect of the methanolic extract of G. glabra root was found to be more in scavenging the free radicals such as DPPH, hydroxyl, H2O2, superoxide, NO, and ABTS when compared to other extracts. The presence of flavonoids and phenol in the plant is responsible for the free radical scavenging effects.Conclusion: This in vitro assessment of the antioxidant properties of the various extracts of G. glabra roots validates and confirms its free-radical scavenging activity. Thus, the present data suggest that the G. glabra root extract can be used as a good source of natural antioxidants for health benefits.
Objective: Tabernaemontana divaricata (Td) is commonly used plants for the treatment of inflammatory, anticancer, and diabetics. The main objective of this study is to assess the antitumor and antioxidant activity of Td in mice model.
Methods:Antitumor activity of TdPf extract (52 µg/ml) is evaluated against Dalton's lymphoma ascites (DLA) tumor mice. After 24 hrs of tumor inoculation, the extract is administered daily for 15 and 60 days. After administration of the lost dose followed by 18 hrs fasting, mice are sacrificed for observation of antitumor activity. Antitumor activity is assessed by monitoring the liver marker enzyme, lipid peroxidation effect on antioxidant enzyme levels, and histopathological evidence.
Results:The results showed that the protein extract of Td animals restored the antioxidant enzymes when compared to the mice of the DLA control group.
Conclusion:The findings indicate that the extract of plant protein has antitumor activity by preventing the lipid peroxidation and thereby promoting the antioxidant systems in DLA induced mice. And hence, it is evident that these extract could be a natural anticancer agent for the human health.
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