Olive (Olea europaea L.) is one of the most economically important crop from east to the west around the world. The aim of this research was to investigate the genetic relationship among 41 olive genotypes, including 11 well-known Turkish cultivars and 30 Azerbaijani olive genotypes using simple sequence repeat (SSR) markers. In this study, 19 SSR markers were amplified 115 polymorphic SSR alleles. The number of polymorphic alleles ranged from 3 to 10 with an average of 6.05. The observed heterozygosity (Ho) varied from 0.05 to 0.93 with an average of 0.63 and expected heterozygosity (He) differed from 0.26 to 0.86 with an average of 0.72. The polymorphism information content (PIC) ranged from 0.23 to 0.85 with a mean of 0.68. A UPGMA cluster analysis grouped olive genotypes into two distinct clusters and both clusters were divided into two subgroups. Similarly, STRU CTU RE analysis assigned olive genotypes into two different gene pools (K = 2) and four gene pools were identified representing the two subgroups by STRU CTU RE analysis for K = 4. The genetic similarity of olive genotypes ranged from 0.36 to 0.95. These results revealed that there was a high genetic variation among 30 Azerbaijani olive genotypes. 'Ayvalık 1'and 'Ayvalık 2' from Azerbaijani olive genotypes were different from Turkish local olive cultivar, "Ayvalık" indicating homonymy. This research also highlighted that Azerbaijani olive genotypes were totally distinct from Turkish olive cultivars demonstrating that these olive genotypes might have been imported to Azerbaijan from different countries other than Turkey. The outcomes of this study indicated that these diverse olive genotypes could be useful for development of new olive varieties in Azerbaijan and future breeding programs between two countries could be enhanced by means of these results.
In the article, genetic diversity of olive samples from Azerbaijan and Turkey, genotyping of natural populations and gene pools with molecular markers, associative mapping, genome analysis, carried out jointly genetic relationships between genotypes of olives and genetics originating from Azerbaijan and Turkey are studied by molecular analysis through their SSR markers. When the research work is successful, the results of this study will be demonstrated the presence of SSR markers to distinguish olive genotypes and further studies on olive production in both countries will be undertaken.
Two different mutations: deletion of 13 exons (from 8th to 20th exons) in one index patient and deletion of 45th exon in the second one were identified by molecular genetical analysis for patients with Duchenne muscle dystrophy diagnosis from different ethnic groups, residing in Azerbaijan. Taking into account reproductive age of parents, the prenatal diagnosis of fetus is recommended for the following pregnancies.
Впервые у жителя Азербайджанской Республики был проведен молекулярногенетический анализ гена дистрофина длиной 2,6 миллион пар нуклеотидов. В двух семьях с диагнозом мышечная дистрофия Дюшенна из различных этнических груп, прожи вающих в разных регионах Азербайджанской Республики, проведен анализ гена дистрофина с использованием комплекса моле кулярногенетических методов. Идентифицировано две различающиеся мутации: делеция большого региона, охваты вающего 13 экзонов (от 8го до 20го экзона) у одного и делеция 45го экзона у другого больного. Учитывая репродуктивный возраст родите лей, предлагается пренатальная диагностика плода во время последующей беременности.
A 9 year old boy with the obvious traits of Duchene muscular dystrophy disease is a resident of Masalli region of Azerbaijan, located in the south-east foothill of Talysh Mountains. Family members such as mother, father and sister of this index patient were examined. To diagnose all members of the family, biochemical analysis was conducted for quantitative analysis of creatine phosphokinase in blood serum and genealogical survey identified inherited disease cystic fibrosis in first cousin of this index patient . Quantitative evaluation of creatine phosphokinase in blood serum was performed for all family members of the index patient ., and identified high values: in index patient (2298 U/L, norm - 38-137 U/L), in his mother (879 U/L, norm - 26-140 U/L) and his sister (852 U/L, norm - 26-140 U/L), whereas his father had normal range values of creatine phosphokinase in blood (53,1 U/L, where norm - 38-137 U/L). Diagnosis of Duchenne muscular dystrophy disease was confirmed in index patient in hemizygous state. His mother and sister were found as heterozygous carriers of the (Duchenne muscular dystrophy) DMD gene. Molecular genetic analysis of the DMD gene (MLPA) identified mutation in the mother and sister of the index patient. Mutation type was nonsense, and classified as pathogenic class. Molecular genetic analysis of the DMD gene showed a gain of mutations, consisting of two copies encompassing exon 03 to 09 in the index patient, mother and his sister. The identified two different mutations of DMD gene in Azerbaijani family: fragment deletion of exon 45 in three sibs from Astara region of Azerbaijan, located in the south-east of the country, and deletion encompassing exons from 8 to 20 in 10-year-old boy in Balakan region, located in the north-west of the Republic Mutation type is a nonsense mutation and classified as pathogenic of class 1. Inherited cystic fibrosis in heterozygous state was additionally identified in the index patient, in father and sister. The screening of identified mutations may serve as a prenatal diagnostic tool to carefully plan the prophylaxis in patients with cystic fibrosis. Furthermore, our studies may serve as a basis for the future investigation of many aberrant molecular mechanisms and regulatory pathways. The study of Duchenne and Becker muscular dystrophy resulted in one of the first successful attempts at reverse genetics, better described as positional cloning, in humans. Discovery and subsequent analysis of the gene mutation that results in the clinical disorder led to the discovery of the encoded protein, dystrophin. This coinage set a precedent for the naming of proteins discovered by positional cloning of human disease genes: for example, huntingtin, emerin, and ataxin.
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