ABSTRAKPada penelitian sebelumnya diketahui bahwa aplikasi patch gingiva mukoadesif β-carotene berfungsi sebagai bahan proteksi efek paparan radiografi panoramik pada hewan coba kelinci New Zealand. Peningkatan jumlah mikronukleus akibat paparan radiografi panoramik dapat dihambat dengan aplikasi patch β-carotene. Sebagai kelanjutan dari penelitian, dilakukan uji klinis terhadap manusia untuk melihat efek proteksi patch tersebut. Sebanyak 20 orang subjek yang akan dipapar radiografi panoramik dimasukkan secara random dalam 2 kelompok. Kelompok I adalah subjek tanpa aplikasi patch gingiva mukoadesif β-carotene dan Kelompok II adalah subjek yang diaplikasi patch sebelum paparan. Setiap subjek dilakukan apusan pada mukosa gingiva anterior rahang atas sebelum dan 10 hari setelah paparan radiografi panoramik. Apusan diwarnai dengan metode modifikasi Feulgen-Rossenbeck. Efek lain dari aplikasi juga dilihat mengenai lama waktu bertahan patch dan hasil radiograf yang dihasilkan. Hasil penelitian menunjukkan bahwa terdapat penurunan jumlah mikronukleus pada kelompok II walaupun tidak berbeda bermakna (p>0,05) terhadap kelompok I. Lama waktu bertahan patch gingiva mukoadesif β-carotene dapat mencapai lebih dari 10 jam dan dilaporkan terasa mengganjal pada awal pemakaian. Hasil analisis gray scale antara radiograf dengan dan tanpa aplikasi tidak menunjukkan perbedaan yang bermakna (p>0,05). Kesimpulan dari penelitian ini adalah aplikasi patch gingiva mukoadesif β-carotene dapat menurunkan jumlah mikronukleus namun tidak secara signifikan.Maj Ked Gi Ind. Desember 2015; 1(2): 186 -192 Kata Kunci: Patch gingiva mukoadesif β-carotene, mikronukleus, paparan radiografi panoramik manusia Abstract: Effect of β-carotene Gingival Mucoadhesive Patch Application at Panoramic Radiography Exposure.According to previous research, β-carotene gingival mucoadhesive patch has a radiation protection effect on New Zealand Rabbits. The increase in micronucleus after panoramic radiography exposure is avoided because of β-carotene gingival mucoadhesive patch application. In order to continue that research, we try the application of β-carotene gingival mucoadhesive patch into human. Twenty subjects who requested to take panoramic radiography were divided into two groups randomly. Group I was without β-carotene gingival mucoadhesive patch application and Group II was given the application before radiography exposure. Each of the subjects was swabbed on anterior maxillary gingiva before radiography exposure and the 10th day after the exposure. The swab was stained using modified Feulgen Rossenbeck method. The adhesive time and the effect of radiograph were also observed. The result shows that there was a decrease in micronucleus number after β-carotene gingival mucoadhesive patch application but there was no significant difference (p>0,05) between Grup I and II. The adhesive time was more than 10 hours and there was uncomfortable taste in the first time application. Gray scale analysis shows no significant difference (P>0,05) between panoramic rad...
Background: Dental digital radiography is more practical and requires fewer doses of radiation than conventional radiography. Because ionizing radiation has a biological effect on exposed tissue, concerns regarding its stochastic effect merit greater attention. In a previous study, it was found that biological changes and increases in the micronucleus occurred after conventional panoramic exposure to gingival crevicular fluid (GCF). Purpose: The purpose of this study is to investigate the difference in biological effects
Aim: Dental imaging has been widely used for diagnosis in dentistry. However, dental X-ray may induce cytotoxicity leading to apoptosis in oral mucosa cells. The present study aimed to observe the maturation pattern of buccal and gingival cells after exposure to X-ray radiation from analog/digital panoramic scanning and cone beam computed tomography (CBCT). Methods: The research samples were 40 subjects who fulfilled the inclusion and exclusion criteria. The subjects were divided into the exposed (patients who received analog/digital panoramic radiography or CBCT) and controlled (patients who had no radiography examinations) groups, with 10 subjects in each group. Exfoliative cytology smears were obtained from buccal mucosa and gingiva before exposure (or on day 0 for the control group) and 10 days later. The cells were stained with the Papanicolaou method. Then, the superficial, intermediate, and parabasal cells were counted in each glass slide. Results: No significant differences (p > 0.05) were observed among all cell types between day 0 and 10 in the control group. Meanwhile, after exposure to three kinds of radiography examinations, the frequency of intermediate cells in buccal mucosa and gingiva increased (p < 0.05), but that of superficial cells decreased (p < 0.05) significantly. No significant difference was found in the parabasal cells (p > 0.05). The frequency differences between intermediate and superficial cells showed no significant difference between the buccal mucosa and gingiva. Conclusion: Analog/digital panoramic radiography and CBCT exposure can induce cytotoxicity by altering the maturation pattern of buccal mucosa cells and gingiva, so it is strongly recommended to only perform these procedures if necessary and avoid repeated exposure to the same patient.
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