SummaryCross-linked dextran beads were chemically modified with Fmoc-/~Ala to give an amino functionalized support suitable for solid-phase peptide synthesis. On this support, a peptide comprising B-cell and T-cell epitopes in tandem was synthesized manually by Fmoc procedure monitoring the coupling reaction by bromophenol blue procedure and standard Kaiser test. The quality of peptide synthesis was verified by RP-HPLC and mass spectrometry. The size and morphologic characteristics of dextran beads were conserved after peptide synthesis. Furthermore, we analyzed immunogenicity in mice of the T-B peptide on dextran beads compared with T-B peptide immunogenicity when administered in CFA/IFA. In both cases, titers were high and there was not a significant difference in antibody titers between groups (p<0.05). But in contrast CFA they are biocompatible and did not induce any adverse reaction at the site of injection. This experiment suggests that the peptides synthesis on dextran beads can be used to raise the immunogenicity of synthetic peptide in vaccines or therapeutics.
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