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The cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures. Here, to comprehensively map cell lineages, we use single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions in the developing and up to 11 distinct anatomical regions in the healthy paediatric and adult human gut. This reveals the existence of transcriptionally distinct BEST4 epithelial cells throughout the human intestinal tract. Furthermore, we implicate IgG sensing as a function of intestinal tuft cells. We describe neural cell populations in the developing enteric nervous system, and predict cell-type-specific expression of genes associated with Hirschsprung’s disease. Finally, using a systems approach, we identify key cell players that drive the formation of secondary lymphoid tissue in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. This catalogue of intestinal cells will provide new insights into cellular programs in development, homeostasis and disease.

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Using hourly records from 51 rain gauges, spanning between 22 and 28 yr, the authors study the diurnal cycle of precipitation over the tropical Andes of Colombia. Analyses are developed for the seasonal march of the diurnal cycle and its interannual variability during the two phases of El Niño–Southern Oscillation (ENSO). Also, the diurnal cycle is analyzed at intra-annual time scales, associated with the westerly and easterly phases of the Madden–Julian oscillation, as well as higher-frequency variability (<10 days), mainly associated with tropical easterly wave activity during ENSO contrasting years. Five major general patterns are identified: (i) precipitation exhibits clear-cut diurnal (24 h) and semidiurnal (12 h) cycles; (ii) the minimum of daily precipitation is found during the morning hours (0900–1100 LST) regardless of season or location; (iii) a predominant afternoon peak is found over northeastern and western Colombia; (iv) over the western flank of the central Andes, precipitation maxima occur either near midnight, or during the afternoon, or both; and (v) a maximum of precipitation prevails near midnight amongst stations located on the eastern flank of the central Cordillera. The timing of diurnal maxima is highly variable in space for a fixed time, although a few coherent regions are found in small groups of rain gauges within the Cauca and Magdalena River valleys. Overall, the identified strong seasonal variability in the timing of rainfall maxima appears to exhibit no relationship with elevation on the Andes. The effects of both phases of ENSO are highly consistent spatially, as the amplitude of hourly and daily precipitation diminishes (increases) during El Niño (La Niña), but the phase remains unaltered for the entire dataset. We also found a generalized increase (decrease) in hourly and daily rainfall rates during the westerly (easterly) phase of the Madden–Julian oscillation, and a diminished (increased) high-frequency activity in July–October and February–April during El Niño (La Niña) years, associated, among others, with lower (higher) tropical easterly wave (4–6 day) activity over the Caribbean.