Soil salinity is a major constraint to soybean production. Five soybean genotypes were grown in pots with hydroponic culture under control and different salt stressed conditions to observe salt tolerance capacity on the basis of phenotypic screening and measure genetic diversity and relatedness among the genotypes. Minimum effects of salinity on root and shoot length was observed in Binasoyben-3, GC840 and Binasoyben-5at different salt stresses. Root dry weight and shoot dry weight of different soybean genotypes under different salt stresses were depicted. The highest reduction in root weight was noted in Binasoybean-1. The same genotypes were used to assess genetic diversity among them with simple sequence repeat (SSR) markers. A total of 33 alleles were detected among 5 soybean genotypes by using 10SSR markers. The number of alleles per locus ranged from 2 to 5, with an average of 3.33 alleles across the 10 loci. Rare alleles were observed at 10 SSR loci with an average of 2.8 alleles per locus. In this experiment, two SSR loci were found to be null alleles. The average values of null allele were 0.2. PIC values ranged from 0.27 in Satt184 to 0.77 in Satt339 with the average value of 0.56.The major allele frequency of the most common allele at each locus ranged from 0.80 in Satt184 to 0.20 in Satt339with a mean frequency of 0.48. The size of the different major alleles at different loci ranged from 173 bp for Satt509 to 407 bp for Satt339. The highest gene diversity (0.80) was observed in loci Satt339 and the lowest gene diversity (0.32) was observed in loci Satt184 with the mean diversity of 0.61. The lowest genetic distance (0.60) was observed in Asset vs Binasoybean-3 and Binasoybean-5 vs Binasoybean-3. The highest genetic distance (1.0) was observed between a numbers of genotype pairs with GC840 vs Asset. The UPGMA cluster analysis led to the grouping of the 5 genotypes into two major clusters. GC840, an advanced line identified to be salt tolerant, together with Binasoybean-5 and Binasoybean-3 clustered in the same sub group. The results from morphological and molecular study suggested that GC840 and Binasoybean-3 are moderately tolerant to salt stress. Progressive Agriculture 30 (1): 1-9, 2019
A study was undertaken to examine the genetic diversity of 12 wheat (Triticum aestivum L.) genotypes, using 4 simple sequence repeats (SSRs). A total of 10 alleles were found. Allele number per locus ranged from 2 to 4 with an average of 2.5. The polymorphic information content (PIC) values ranged from 0.2755 to 0.5411 with an average of 0.3839. The average gene diversity over all SSR loci for the 12 wheat genotypes was 0.4688, ranging from 0.3299 to 0.6042. Cluster analysis based on microsatellite allelic diversity discriminated the varieties into different clusters. Genetic diversity was the highest between variety Gourab and Akbar as well as Gourab and BAW-1064, showing a genetic distance value of 0.4697. The genetic distance was lowest between Balaka and Aghrani as well as Triticale and BAW-1036. Positive correlations were found between gene diversity, number of alleles, the allele size range and the types of repeat motif of microsatellite markers. It was found from this study that microsatellite markers could characterize and discriminate all of the genotypes. More primers should be used for saturation of different regions in further studies. Bangladesh J. Agril. Res. 37(3): 389-398, September 2012 DOI: http://dx.doi.org/10.3329/bjar.v37i3.12082
Three advanced lines (SB02, SB05, SB07) along with one tolerant (Lokon) and one susceptible check (Asswt) of soybean (Glycine max L.) were assessed for salt tolerance in terms of morpho-physiological traits and molecular markers (SSR). The experiment was conducted at seedling stage with four salinity treatments namely 0, 8, 12 and 16 dSm-1 following Completely Randomized design. All the genotypes displayed considerable reduction in their morphological traits, least affecting the tolerant one. None of the genotypes were survived at 12 and 16 dSm-1 stress condition. Among the lines tested, SB-02 and SB-05 were identified as salt tolerant at 8 dSm-1 based on salinity susceptibility index (SSI) scoring. These genotypes suffered less in reduction of leaf chlorophyll content (SPAD) and increase of Na+/K+ than the susceptible genotypes. For all the traits viz. shoot length, root length, total length, shoot fresh weiht, root fresh weight, shoot dry weight, root dry weight, total fresh weight, total dry weight, percent live leaves, chlorophyll content and Na+/K+ ratio, the phenotypic coefficient of variation (PCV) was higher than that of genotypic coefficient of variation (GCV). All the traits studied showed medium to high heritability ranging between 43.81% (SPAD) to 96.65% (shoot length). The genotypes were grouped into two clusters considering both Euclidian distance and Unweighted Pair Group Method with Arithmetic Mean analysis. Lokon, SB-02 and SB-05 are on the same cluster as tolerant, and SB-7 and Asset on the other as susceptible to salt stress. The molecular pattern using by SSR marker displayed an average number of 3.33 alleles per locus with PIC (Polymorphism Information Content) values ranged from 0.2688 (sat_655 and satt728) to 0.7680 (sat_210). The highest gene diversity was observed in sat_210 and satt237 and the lowest in sat_655 and satt728 with a mean diversity of 0.5733. The genotypes Lokon, SB-02 and SB-05 could be suggested as a potential germplasm source of QTL (Quantitative Trait Loci) analysis for the development of salt tolerant soybean variety. Ann. Bangladesh Agric. (2020) 24(2): 33-46
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