Premise of the study:We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador.Methods and Results:Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii.Conclusions:We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.
Annona deceptrix (Annonaceae) is a wild endemic endangered species. This species presents relevant attributes that man can use and be considered as a consumption option, but, its natural populations have a high degree of vulnerability, due to the loss their ecosystems caused by man. This study aimed to isolate and characterize SSR loci to determine the genetic diversity in A. deceptrix and other Annonaceae. We identified simple sequence repeat (SSR) regions in a microsatellite library from A. deceptrix. 22 polymorphic Simple Sequence Repeat (SSR) loci were isolated using new-generation kit Nextera of miseq illumine technology. The mean number of alleles per locus ranged from 2 to 8. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.93, respectively. These are the first microsatellite markers developed for A. deceptrix. All SSR markers developed are promising candidates for analyzing genetic diversity within or between natural populations, also of analyzing pool genic in other species of Annona genus.
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