Inflammatory reactions give rise to a series of processes and symptoms that can cause pain and damage to nearby cells. Inflammatory reactions are mediated by inflammatory and proinflammatory cytokines. NSAID class (non-steroidal anti-inflammatory drug) drugs have been developed to inhibit the inflammatory process by suppressing COX-1 and / or COX-2 enzyme activity. However, this drug can cause gastric mucosal irritation. the ethanol extract of Annona muricata leaf has an analgesic and anti-inflammatory effect[3].The purpose of this research is to know analgesic and antiinflamatory activity from ethanol extract and essential oil of Annona muricata leaf.The method used was the study of in silico by molecular docking of active compounds in ethanol extracts and essential oils to the COX-1, COX-2, phospolipase-A, and TNF-alpha receptor molecules as target molecules. The molecular docking results show that Annona muricata ethanol extract can act as an anti-inflammatory and analgesic, especially through strong bonds between "rutine" compounds with COX-2 receptors with affinity -10 kcal / mold and "quercetin" with affinity bindings -9.8 kkal.mol, better than dexametason (binding affinity -9.6 kcal / mol). Keywords:Inflamation, COX-1, COX-2, phospolipase-A, TNF-alfa, docking
Pendahuluan: Inflamasi akibat kerusakan oksidatif yang dipicu hiperglikemia berperan terhadap progresivitas diabetes melitus (DM). Interleukin-10 (IL-10) dan CD8+ berpengaruh terhadap proses inflamasi. Abelmoschus manihot (L.) Medik memiliki efek antiinflamasi dan antioksidan yang diharapkan dapat menurunkan inflamasi pada patofisiologi DM. Penelitian ini bertujuan untuk mengetahui efek ekstrak etanol Abelmoschus manihot (L.) Medik terhadap persentase IL-10 dan CD8+ tikus model DM.Metode: Tikus Sprague Dawley jantan, 4-6 minggu dikelompokkan menjadi kelompok kontrol normal (KN), kelompok kontrol diabetes melitus (KDM), kelompok ekstrak etanol daun gedi merah (EEDGM) 200 mg/kgBB, EEDGM 400 mg/kgBB dan EEDGM 800 mg/kgBB (n=5). Hewan coba diinduksi diet tinggi lemak-fruktosa (DTLF) selama 10 minggu dan Streptozotocin (STZ) dosis rendah 25 mg/kgBB intraperitoneal dosis ganda pada minggu ke 4. EEDGM 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB diberikan selama 4 minggu. Pengukuran persentase CD8+ dan IL-10 menggunakan flowcytometry. Analisa data menggunakan One Way Anova dilanjutkan dengan uji LSD (p<0,05).Hasil: Induksi DTLF dan STZ pada kelompok KDM menurunkan persentase IL-10 dan meningkatkan persentase CD8+ dibandingkan KN (p<0.05). Pemberian EEDGM dosis 400 mg/kgBB dan 800 mg/kgBB signifikan meningkatkan persentase IL-10 berturut-turut sekitar ½ dan ¾ dibandingkan kelompok KDM (p<0,05). Pemberian EEDGM dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB menurunkan persentase CD8+ berturut-turut 1/5, 1/3 dan 1/10 dibandingkan kelompok KDM (p<0,05).Kesimpulan: Pemberian EEDGM meningkatkan persentase IL-10 dan menurunkan persentase CD8+ tikus model diabetes
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