In the present study, phytofabricated selenium nanoparticles (PF-SeNPs) were prepared from aqueous fruit extract of Emblica officinalis in a facile, green, economic, tactic and eco-friendly way. The aqueous fruit extract of E. officinalis was found to be rich with various secondary metabolites including phenolics (59.18 ± 2.91 mg gallic acid equivalents/g), flavonoids (38.50 ± 2.84 mg catechin equivalents/g), and tannins (44.28 ± 3.09 mg tannic acid equivalents/g) and determined that highly appropriate for the biosynthesis of nanoparticles. The facile phytofabrication of PF-SeNPs was confirmed by UV-visible and FTIR spectroscopic analysis. The XRD pattern and Raman spectroscopy showed that synthesized PF-SeNPs were amorphous in nature. The Zeta potential analysis confirmed that PF-SeNPs were negatively charged (-24.4 mV). The DLS analysis revealed that PF-SeNPs were in nano size and less aggregated with poly-dispersity index of less than 0.2. The SEM images depicted that PF-SeNPs were spherical in shape. The EDX analysis revealed that PF-SeNPs were constituted with Se (61.60%), C (29.96%), and O (4.41%). The HR-TEM analysis determined that PF-SeNPs were in nano size with an average diameter of 15–40 nm. The PF-SeNPs have offered fascinating bio-potential applications, such as antioxidant, antimicrobial and biocompatibility. They have also exhibited dose-dependent free radical scavenging activity, and EC50 was determined as 15.67 ± 1.41 and 18.84 ± 1.02 μg/mL for DPPH and ABTS assays, respectively. The PF-SeNPs has also shown the wide range of antimicrobial activity on foodborne pathogens, and it was found to be highly efficient on fungi followed by Gram-positive and Gram-negative bacteria. The biocompatibility of PF-SeNPs was assessed in N2a cells with much higher IC50 value (dose required to inhibit 50% of cell viability) compared to sodium selenite. Also, mitochondrial membrane potential (MMP) and caspase-3 were much less altered on treatment of PF-SeNPs related to sodium selenite. The cytotoxic studies clearly determined that PF-SeNPs was much less toxic and safer related to sodium selenite. Thus, PF-SeNPs could find suitable application as antioxidant and antimicrobial agent in food, biomedical, and pharmaceutical industry.

No abstract

The antifungal activity of essential oils from clove, cedar wood, Cymbopogon species, peppermint, Eucalyptus and neem were tested for their efficacy against nine Fusarium species,

Ascomycetous fungi are found associated with a wide variety of substrates which range from fresh water to marine ecosystems, tropical to temperate forest soils and deserts, throughout the world over. These demystifying fungi exist as endophytes, pathogens and saprobes. They have been studied due to their ability to contaminate foods and feedstuffs, causing an elaboration of mycotoxins. The objectives of the study included extensive analyses of the morphological features of fungi, especially Aspergilli, which have been presented while studying them on specific mycological media. It is also an elaborate compilation of substantive macro- and micro-morphological characterization of different Aspergilli isolated from the spice Foeniculum vulgare used in India and other countries in the world. Further, a first of its kind attempt has been made to study their relative abundance and frequency of occurrence, molecular phylogeny and genetic relatedness to characterize the Aspergilli into specific sections, groups and clades. Single nucleotide polymorphism (SNP) analysis was carried out to evaluate the functional consequences of nucleotide variations, synonymous and non-synonymous mutations in the protein structure. The study resulted in a total of 3,506 Aspergillus isolates, which were obtained from seventy (70) fennel samples, representing 14 Aspergillus species. The two most frequently found species were A. niger and A. flavus with a relative abundance of 32.24 and 11.63%, respectively. The taxonomy and current placements have been reappraised with suggestions and prospects for future research from six sections namely Terrei, Flavi, Fumigati, Nidulantes, Nigri, and Versicolores. In addition, a total number of 27 isolates were studied and deposited at the National Centre for Biotechnology Information (NCBI) and five Aspergillus species have been identified and are being reported for the first time from the fennel seeds, based on partial sequence analysis of the official fungal barcode namely, Internal Transcribed Spacer (ITS) and a functional gene, beta tubulin gene locus, coupled with phenotypic characterization. SNPs for specific DNA regions have been used to identify variants in Aspergilli obtained from Indian fennel seeds for the first time. The need for a polyphasic approach of morphological identification and genetic characterization of Aspergilli from Foeniculum vulgare is addressed and presented here in adequate detail. Our current work makes extensive use of partial beta-tubulin gene sequences analyses to evaluate the association between SNPs in five Aspergillus species sections.

Maize (Zea mays L.) is the third most popular Poaceae crop after wheat and rice and used in feed and pharmaceutical sectors. The maize silk contains bioactive components explored by traditional Chinese herbal medicine for various pharmacological activities. However, Fusarium graminearum, Fusarium verticillioides, Trichoderma atroviride, and Ustilago maydis can infect the maize, produce mycotoxins, hamper the quantity and quality of silk production, and further harm the primary consumer’s health. However, the defense mechanism is not fully understood in multiple fungal infections in the silk of Z. mays. In this study, we applied bioinformatics approaches to use the publicly available transcriptome data of Z. mays silk affected by multiple fungal flora to identify core genes involved in combatting disease response. Differentially expressed genes (DEGs) were identified among intra- and inter-transcriptome data sets of control versus infected Z. mays silks. Upon further comparison between up- and downregulated genes within the control of datasets, 4,519 upregulated and 5,125 downregulated genes were found. The DEGs have been compared with genes in the modules of weighted gene co-expression network analysis to relevant specific traits towards identifying core genes. The expression pattern of transcription factors, carbohydrate-active enzymes (CAZyme), and resistance genes was analyzed. The present investigation is supportive of our findings that the gene ontology, immunity stimulus, and resistance genes are upregulated, but physical and metabolic processes such as cell wall organizations and pectin synthesis were downregulated respectively. Our results are indicative that terpene synthase TPS6 and TPS11 are involved in the defense mechanism against fungal infections in maize silk.