This research aims to fabricate the novel bio-hybrid composite scaffold from mesoporous bioactive glasses/silk fibroin/chitosan (MBGs/SF/CS) for use in tissue engineering applications. MBGs/SF/CS composite scaffolds were successfully fabricated using freezing and lyophilization process. Two types of mesoporous bioactive glasses which were irregular and spherical shape were dispersed in the silk fibroin/chitosan based scaffolds in order to improve the mechanical strength and bioactivity. SEM observation showed the interconnected pores with pore size from 100 to 300 µm. XRD and FTIR exhibited the present of silk fibroin, chitosan, and MBGs in composite scaffolds. The incorporation of MBGs in SF/CS scaffolds significantly increased the compressive strength of scaffolds. The composite scaffolds were immersed in the simulated body fluid (SBF) for in vitro bioactivity test. The in vitro bioactivity results indicated that the MBGs/SF/CS induced hydroxycarbonate apatite (HCA) formation while there was no change for SF/CS scaffolds. Furthermore, mesoporous bioactive glass with micro-spherical particles (MBGMs) which easily dispersed in SF/CS solution during the fabrication of scaffolds as compared to mesoporous bioactive glass with irregular shape (MBGs). The results showed that MBGs/SF/CS composite scaffolds could be useful composite scaffolds for tissue engineering applications.
Human adipose-derived stem/stromal cells (hASCs) have been popularly studied as cell-based therapy in the field of regenerative medicine due to their ability to differentiate into several cell types. In this study, in order to improve the mechanical strength and bioactivity of scaffolds for bone tissue engineering, three types of mesoporous bioactive glasses with different shapes and compositions were dispersed in the silk fibroin/chitosan (SF/CS)-based scaffolds, which were fabricated with a combination of freezing and lyophilization. The characteristic and physical properties of these composite scaffolds were evaluated. The biocompatibility was also assessed through hASCs in vitro tests. Both Alamar Blue® and Live/Dead assay® revealed that the spherical mesoporous bioactive glass doped scaffolds enhanced cell viability and proliferation. Furthermore, the addition of spherical mesoporous bioactive glass into SF/CS scaffolds encouraged hASC osteogenic differentiation as well. These results suggested that this composite scaffold can be applicable material for bone regeneration. K E Y W O R D S bioactive glass, chitosan, composite scaffold, human adipose-derived stem/stromal cells, silk fibroin, tissue engineering 2780 | PHETNIN ET al. How to cite this article: Phetnin RB, Suksaweang S, Giannasi C, et al. 3D mesoporous bioactive glass/silk/ chitosan scaffolds and their compatibility with human adipose-derived stromal cells.
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