Background: Some pathogenic bacteria cause diseases under certain conditions, such as entry through the skin via a cut. Staphylococcus and Pseudomonas can be commonly found on the skin; yet, these species can potentially initiate skin infections. Considering the importance of these bacteria in skin infections and based on the world attraction to traditional treatment, the present study was conducted to determine the antibacterial effect of Pimpinella anisum polar extract on standard strains of Pseudomonas aeruginosa and Staphylococcus aureus. Methods: Antimicrobial effect of anisum's polar extract was determined by the well diffusion agar and microtitr plate for determining the minimum bactericidal concentration (MBC) and minimum inhibitory concentrations (MIC) on Staphylococcus aureus, Pseudomonas aeruginosa. Results: Inhibition zones were recorded for both bacteria in this study. The MIC/MBC assay showed that Pseudomonas aeruginosa bacteria had the highest susceptibility to the anisum's polar extract. Conclusions: Considering the antibacterial effects of the Pimpinella anisum extract, the extract of this plant can be studied as a treatment for skin infections.
Skin is the largest organ in vertebrates that is of great importance and performs as a protective barrier against the external world. It serves various functions such as protecting against external insults, fluid homeostasis, self-healing, and sensory detection. Skin disorders impose major financial and social burden. Therefore, the regeneration potential of the skin is an important area of research in tissue engineering (TE). This potential is due to the stem cells; they play a vital role in the skin regeneration process, since one of the main characteristics of the stem cells is their ability to differentiate into the organ specific specialized cells. Numerous factors such as growth factors, topography, etc. are involved in stem cell differentiation. In the current study, primary human keratinocytes were isolated from the foreskin samples and cultured. Then, the substrate was developed using PDMS (polydimethylsiloxane) silicon following keratinocyte fixation. This substrate can be used in further stem cell culture and differentiation studies.
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