The bovine mastitis caused by coagulase negative staphylococci (CNS) has increased in many herds of urban and rural areas of
India. Emergence of multi drug resistant bacteria has further made its management more complex and serious. Therefore,
innovation of novel specific drug for the treatment of disease caused by particular organism remained to be a challenge. Hence, in
the present study a bacterium was isolated from milk of the cow with bovine mastitis and was identified as S. saprophyticus, 44
pathways of S. saprophyticus retrieved (KEGG) from web server were found to be non homologous to the host Bos taurus, out of
which 39 pathways were found to be in cytoplasm, 2 in cell wall and 3 in the cell membrane. The knowledge of the present study
could make the drug discovery easier which have high affinity to the target site of the causative organism.
The study was carried out to investigate the prevalence and antimicrobial susceptibility of Coagulase-Negative Staphylococci isolated from Bovine Mastitis in and around Dharwad region. A total of 310 samples were screened and 180 confirmed Coagulase-Negative Staphylococci were obtained. The antimicrobial susceptibility of Coagulase-Negative Staphylococci against 10 antimicrobial agents was tested using the disc diffusion method. The highest numbers of Coagulase-Negative Staphylococci were susceptible to ceftriaxone 83.88% followed by cefotaxime 79.41%, methicillin 76.47%, ciprofloxacin 73.52%, erythromycin 70.05%, amikacin 66.11%, gentamycin 42.94%, amoxicillin 36.76%, ampicillin 29.41%, and the lowest susceptibility was shown in penicillin 23.23% . The results indicated that the increase in prevalence and antibiotic resistance pattern of the Coagulase-Negative Staphylococci isolated from bovine mastitis exhibited the highest degree of susceptible to ceftriaxone of all the tested antimicrobial agents.
The purpose of this study is to identify and analyse the putative promoter motifs in the bovine herpes virus which causes several
diseases in cattle worldwide including bovine mastitis with large economic impact on dairy industry. Bovine mastitis caused due
to virus is often neglected as bacterial infections are held mainly responsible for the disease. Therefore, in this in silico investigation
with all the existing experimental data a total of 147 promoter were identified along with their sequences from three genome viz
bovine herpes virus 1 (BHV), bovine herpes virus 4 and bovine herpes virus 5, out of which 39 promoters were from bovine herpes
virus 4 (BHV 4), 95 from BHV1 and 13 from BHV5 and it was observed that BHV1 and BHV5 have a close evolutionary history.
However, they belong to the same subfamily and size of the genome and GC% of BHV1 and BHV5 was almost equal and very high
compare to that of BHV4. This analysis may help in designing the live attenuated vaccine against BHV causing bovine mastitis that
reduces the incidence of bovine mastitis. Identification of promoters may also help in designing of expression vectors which help in
better understanding of the regulation of gene expression. In the era of large genomics and proteomics prediction of promoters in
the whole genome is crucial for the advancement of drug discovery and gene therapy.
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