This research investigated the structural characteristics and enzymolysis kinetics of rice protein which was pretreated by energy-gathered ultrasound and ultrasound assisted alkali. The structural characteristics of rice protein before and after the pretreatment were performed with surface hydrophobicity and Fourier transform infrared (FTIR). There was an increase in the intensity of fluorescence spectrum and changes in functional groups after the pretreatment on rice protein compared with the control (without ultrasound and ultrasound assisted alkali processed), thus significantly enhancing efficiency of the enzymatic hydrolysis. A simplified kinetic equation for the enzymolysis model with the impeded reaction of enzyme was deduced to successfully describe the enzymatic hydrolysis of rice protein by different pretreatments. The initial observed rate constants (Kin,0) as well as ineffective coefficients (kimp) were proposed and obtained based on the experimental observation. The results showed that the parameter of kin,0 increased after ultrasound and ultrasound assisted alkali pretreatments, which proved the effects of the pretreatments on the substrate enhancing the enzymolysis process and had relation to the structure changes of the pretreatments on the substrate. Furthermore, the applicability of the simplified model was demonstrated by the enzymatic hydrolysis process for other materials.
The goals of this exploration were to find out the optimum conditions of ultrasound assisted extraction (UAE) of L. Barbarum crude polysaccharides. Furthermore, to research the impacts of three multi-frequency ultrasound assisted extraction modes on the yield of L. Barbarum crude polysaccharides. The results showed that by applying the traditional single-frequency UAE mode, the optimum extraction time was 30 min, extraction temperature of 60 °C, and solid/liquid ratio of 20 g/600 mL, at a power density of 300 W/L, and ultrasound frequency of 28 kHz. Secondly; a comparison was carried out between three UAE modes using the optimum extraction conditions obtained previously. The energy aggregation counter flow dual-frequency UAE mode gave the highest yield of 38.93% of crude polysaccharides. Followed by the Dpposite-sit dual-frequency UAE mode and the energy aggregation counter flow single-frequency UAE mode with yields of 33.60%, and 26.38% of crude polysaccharides, respectively. As a result the ultrasound assisted extraction with dual-frequency mode is more effective for the extraction of L. Barbarum crude polysaccharides. Furthermore, the yield of crude polysaccharides increased by 73.41% using the dual-frequency ultrasound extraction compared to traditional hot water extraction.Practical Application: The extraction of Lycium Barbarum crude polysaccharides was examined utilizing ultrasound assisted extraction technique. The optimum extraction conditions were studied and applied on three extraction modes to discover the best extraction procedure.
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