No abstract

cited, fail to show any deficiency of this enzyme in leukemic cells and the elevated UDPG in myeloid leukemic cells remains unexplained. There appeared to be no correlation with therapy or differential count, although in two cases of chronic myelocytic leukemia studied prior to, and during treatment, there was an increase of the specific activity.

No abstract

A~t-The interaction of concanavalin A with a comprehensive series of carbohydrate-bovine serum albumin conjugates was investigated by agar gel diffusion and quantitative precipitation techniques. These studies showed that when conjugated to bovine serum albumin, those sugars which inhibited concanavalin A-polysaccharide interaction formed a precipitate with concanavalin A. The unexpected reactivity of the p-phenylazo /3-D-glucopyranoside-bovine serum albumin conjugate is rationalized on the basis of inhibition data employing several aryl and alkylglycosides.

Abstraet-Laminaribiose (3-o-/3-D-glucopyranosyl-D-glucose) was coupled to bovine serum albumin (BSA) by an azophenyl linkage to provide the synthetic antigen BSAp-phenylazo-/3-1aminaribioside. The behavior of antisera prepared in rabbits immunized with the /3-1aminaribiosyl conjugate was examined by immunodiffusion, quantitative precipitation and hapten inhibition. Anticonjugate absorbed with carrier protein showed the greatest reactivity with the homologous /3-1aminaribiosyl-BSA antigen, but also showed some cross precipitation with /3-cellobiosyl, /3-sophorosyl and /3-gentiobiosyl-BSA conjugates. Glucobioses linked through the /3-(1-* 2), /3-(1-* 3), /3-(1-* 4) and /3-(1-* 6) positions, as well as laminaridextrins and tri and tetrasaccharides of/3-1inked glucose possessing a laminaribiose moiety either at a nonreducing end location or at a subterminal location, were assayed for their ability to inhibit antilaminaribioside precipitation. Hapten inhibition data showed anticonjugate to possess a high degree of specificity directed against the terminal nonreducing/3-1aminaribiosyl end group.