Hyperspectral microscopy in biology and minerals, unsupervised deep learning neural network denoising SRS photos: hyperspectral resolution enhancement and denoising one hyperspectral picture is enough to teach unsupervised method. An intuitive chemical species map for a lithium ore sample is produced using k -means clustering. Many researchers are now interested in biosignals. Uncertainty limits the algorithms’ capacity to evaluate these signals for further information. Even while AI systems can answer puzzles, they remain limited. Deep learning is used when machine learning is inefficient. Supervised learning needs a lot of data. Deep learning is vital in modern AI. Supervised learning requires a large labeled dataset. The selection of parameters prevents over- or underfitting. Unsupervised learning is used to overcome the challenges outlined above (performed by the clustering algorithm). To accomplish this, two processing processes were used: (1) utilizing nonlinear deep learning networks to turn data into a latent feature space ( Z ). The Kullback–Leibler divergence is used to test the objective function convergence. This article explores a novel research on hyperspectral microscopic picture using deep learning and effective unsupervised learning.