Background: MSCs are known to secrete abundant CCL2, which plays a crucial role in recruiting TAMs, promoting tumor progression. It is important to know whether disrupting MSC-derived CCL2 affects tumor growth. Methods: Murine bone marrow-derived MSCs were characterized by their surface markers and differentiation abilities. Proliferation and migration assays were performed in order to evaluate the functions of MSCs on cancer cells. CCL2 expression in MSCs was reduced by small interfering RNA (siRNA) or completely disrupted by CRISPR/Cas9 knockout (KO) approaches. An immune-competent syngeneic murine model of prostate cancer was applied in order to assess the role of tumor cell- and MSC-derived CCL2. The tumor microenvironment was analyzed to monitor the immune profile. Results: We confirmed that tumor cell-derived CCL2 was crucial for tumor growth and MSCs migration. CCL2 KO MSCs inhibited the migration of the monocyte/macrophage but not the proliferation of tumor cells in vitro. However, the mice co-injected with tumor cells and CCL2 KO MSCs exhibited anti-tumor effects when compared with those given tumor cell alone and with control MSCs, partly due to increased infiltration of CD45+CD11b+Ly6G− mononuclear myeloid cells. Conclusions: Disruption of MSC-derived CCL2 enhances anti-tumor functions in an immune-competent syngeneic mouse model for prostate cancer.
Tetralogy of Fallot is a common cyanotic congenital heart disease. Early surgery treatment gives patients a chance to have normal physical and mental health due to preventing complications of cyanosis and even death as a natural history of tetralogy of Fallot. This study aimed to show short-term outcomes (including the time of postoperative hospital stay and 30 days after discharge) of tetralogy of Fallot complete repair at the Department of Pediatric Cardiac Surgery, Cho Ray Hospital. A retrospective study was performed on the dataof medical documents of 38 patients, who underwent complete repair for tetralogy of Fallot with full sternotomy and cardiopulmonary bypass from March 2017 to December 2019, following a collection form. The results showed the average age and weight were 33.89±13.02 months and 11.77±2.48 kg. The cardiopulmonary bypass time was 147.74±12.48 mins, the aortic cross-clamp time 104.84±9.73 mins, the mechanical ventilation time 31.14±10.75 hours, the duration of stay in the intensive care unit 58.03±13.56 hours, the postoperative hospital stay time 10.12±1.5 days, and the mortality rate 2.6%. The study concludes that the short-termsurgical outcome of complete repair for tetralogy of Fallot in lightweight children is safe and effective with a low mortality rate.
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