a b s t r a c t a r t i c l e i n f oArticle history: Received 9 January 2016 Received in revised form 26 July 2016 Accepted 29 July 2016 Available online xxxx An integrative biomarker approach was employed to evaluate the environmental quality of the north coast of Shandong Peninsula along the southern Bohai Sea of China, where pollution is an imminent threat due to rapid urbanization and industrialization. A battery of biomarkers and the metal bioaccumulation in tissues of native oyster Crassostrea gigas were measured under field conditions. Integrative biomarker index (IBR) and metal body burden were calculated to differentiate the pollution status of seven sampling sites. According to our results, Xinzhuang (XZ) site was the most severely contaminated, with the highest IBR value of 3.58, while the lowest IBR value (0.04) was obtained at Penglai (PL). Such an integrated biomarker approach was proved as a useful method for environmental quality assessment in the study area.
• An ultra performance liquid chromatography-tandem mass spectrometry was developed for determining Semicarbazide, with short time, high sensitivity and accurate characterization.• The temporal and spatial distribution of Semicarbazide in seawater was measured and the highly polluted areas identified and traced.• The distribution of Semicarbazide in sediment and shellfish was also analyzed.• An early warning value 0.05 μg/L in seawater was deduced. A verified method for measuring Semicarbazide (SEM) in seawater, sediments, and shellfish was developed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A total of 30 stations were radially distributed in Jincheng and Sishili Bays in the Bohai and Yellow Seas, and 1025 monitoring data were collected in 41 voyages, 615 seawater samples, 320 sediment samples and 90 shellfish samples. The concentration ranged from 0.011 μg/L to 0.093 μg/L and 0 to 0.75 μg/kg in seawater and shellfish respectively, but SEM in sediment was all below the limit of detection. Temporal and spatial distribution of SEM was investigated using multivariate analysis to estimate the degree of SEM pollution. Based on the SEM concentration in the three sample types, together with our previous findings, early warning values were deduced for SEM in seawater, and the developed method overcame shortcomings with existing technologies. The results may be helpful to draft national baseline values for SEM in seawater and sediments, and provide a scientific basis for assessing the impacts of SEM on marine ecology and human health.
G R A P H I C A L A B S T R A C T a b s t r a c t a r t i c l e i n f o
Here, we determined the complete mitogenome sequence of Euspira gilva (Littorinimorpha: Naticidae). The complete mitochondrial genome is circular and 16,119 bp in length, containing 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22 transfer RNA genes. All PCGs and rRNA genes are encoded on the heavy strand while tRNA genes are distributed in both the strands. The overall nucleotides base composition of the heavy strand is A (31.80%), G (14.72%), C (13.72%), and T (39.76%), with an A þ T bias (71.56%). All of the PCGs had ATG as their start codon. The common termination codon is TAA, except for nd4l terminated with TAG. The 22 tRNA genes could be folded into a typical cloverleaf secondary structure. As seen from the phylogenetic tree, E. gilva has a more close relationship with Neverita didyma and Glossaulax reiniana.
ARTICLE HISTORY
2020) The complete mitochondrial genome of the green alga Chloroidiumsp. UTEX3077 (Watanabea clade, Trebouxiophyceae), Mitochondrial DNA Part B, 5:1, 671-672,
ABSTRACTThe complete mitochondrial genome of Chloroidium sp. UTEX 3077 was determined in this study. The circular genome was 90,774 bp in length with the GC content of 38.8%. It contained 30 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes and 2 ribosome RNA (rRNA) genes. A phylogenetic analysis based on the 8 mitochondrial genomes of Trebouxiophyceae indicated that Chloroidium sp. UTEX 3077 grouped with Chlorellales.
ARTICLE HISTORY
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