Researches on low-molecular organic compounds are concentrated in amino acid and Humic acid chelating fertilizer, but few in sorbitol chelated calcium. In this study, selecting sorbitol as chelating agent, the technological conditions of chelating sorbitol with calcium and the effect of sorbitol chelated calcium on potato were studied. The impact factors on the stability of the fertilizer were investigated, including sorbitol dosage, pH value, chelating time and temperature. Then a field experiment was conducted on potato. Three treatments included water sprayed as a control, calcium nitrate and sorbitol chelated calcium sprayed. Each treatment had three replicates. The results showed that by orthogonal experiments, the sorbitol dosage has the greatest influence on the stability of the chelating system. The optimum conditions for the synthesis were determined as follows: The sorbitol dosage of 12 g, the chelating temperature of 50°C, the pH of 4 and the chelating time of 45 min. Compared with those of potatoes fertilized with inorganic calcium nitrate, the yield of potatoes sprayed with sorbitol chelated calcium increased by 14.23% and their marketable tuber percentage increased by 3.58%; the nitrate nitrogen content in potatoes decreased by 11.89%; the accumulation of phosphorus and potassium in potato tubers increased. Generally, the application effect of our prepared sorbitol chelated calcium on potato was remarkable.

Background:In order to detect the antimicrobial mechanism of combined treatment of cinnamon oil and gamma irradiation (GI), the membrane fatty acids and proteins characteristics of Shewanella putrefaciens (S. putrefaciens) treated with cinnamon oil and GI, and the distribution of cinnamon oil in S. putrefaciens were observed in this study. Results:The membrane lipid profile of S. putrefaciens was notably damaged by treatments of cinnamon oil and the combination of cinnamon oil and GI, with significantly fatty acids decrease in C14:0, C16:0, C16:1, C17:1, C18:1 (p < 0.05). The SDS-PAGE result showed that GI did not have obvious effect on membrane proteins (MP), but GI combined with cinnamon oil changed the MP subunits. Cinnamaldehyde, the main component of cinnamon oil, can not transport into S. putrefaciens obviously. It was transformed into cinnamyl alcohol in the nutrient broth with the action of S. putrefaciens. This indicated that the antimicrobial action of cinnamon oil mainly happened on the membrane of S. putrefaciens. Conclusion:Cinnamon oil could act on the membrane of S. putrefaciens with the damage of fatty acids and proteins, and GI would increase the destructive capability of cinnamon oil on the membrane fatty acids and proteins of S. putrefaciens.

The aims of this study were to observe the antimicrobial effect and mechanism of cinnamon oil combined with gamma radiation on Gamma radiation increased the antimicrobial activity of cinnamon oil, and the relative radiation sensitivity of gamma radiation on was increased by cinnamon oil. Gamma radiation significantly increased the changes of bacterial morphology, intra-adenosine 5'-triphosphate (intra-ATP) and extra-ATP concentrations and pH value of treated cinnamon oil. Although, gamma radiation used alone didn't damage the bacterial morphology and ATP concentrations significantly. Gamma radiation assisted cinnamon oil to damage the cell permeability and integrity of thus the combination of cinnamon oil and gamma radiation showed a better antimicrobial activity than used alone.

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Sorbitol has the effect of promoting nutrient absorption, so it has the potential to produce excellent fertilizers. In this study, sorbitol calcium chelate was synthesized using sorbitol as the chelating agent. The effects of sorbitol calcium chelate on peanut were investigated, with the goal of promoting peanut yield and improving the utilization of calcium fertilizer. Field experiments were carried out in Jimo, Shandong Province, China. Four sorbitol calcium chelate concentrations (0.4, 0.8, 1.6 and 2.4 g L 1 ) and two sorbitol non-chelated calcium concentrations (1.6 and 2.4 g L 1 ) were tested. Compared with the corresponding sorbitol non-chelated calcium fertilizer treatments, the yield of peanuts increased by 10.0 and 1.7% under sorbitol chelated calcium concentrations of 1.6 and 2.4 g L 1 , respectively, besides, the calcium and potassium contents of the seed kernel increased by 32.2 and 55.8%, under sorbitol chelated calcium concentrations of 1.60 and 2.40 g L 1 , respectively. Sorbitol calcium chelate improved peanut yield and promoted the transformation of sugars to fat in the seed kernel, the 1.6 g L 1 concentration produced the best results. Compared with the blank control, the 1.60 g L 1 sorbitol chelated calcium fertilizer treatment increased peanut yield by 28.6%, fruit number per plant by 46.8%, 100-kernel weight by 20.4%, full fruit number per plant by 55.3%, fat content by 5.0%. In conclusion, sorbitol chelated calcium fertilizer was superior to sorbitol nonchelated calcium fertilizer under the experimental conditions used herein.