Study Group* the establishment and maintenance of pregnancy in humans proceed through a continuous change of biochemical and biophysical processes. It requires a constant interaction between the fetus and the maternal system. The present prospective study aims to elucidate changes in salivary proteome from the early to middle stages of term pregnancy, and establishing an expressional trajectory for modulated proteins. To date, a comprehensive characterization of the longitudinal salivary proteome in pregnancy has not been performed and it is our immediate interest. In the discovery phase, maternal saliva (n = 20) at 6-13, 18-21, and 26-29 weeks of gestation was analyzed using level-free proteomics (SWATH-MS) approach. The expression levels of 65 proteins were found to change significantly with gestational age and distributed into two distinct clusters with a unique expression trajectory. The results revealed that altered proteins are involved in maternal immune modulation, metabolism, and host defense mechanism. Further, verification of 12 proteins was employed using targeted mass spectrometry (MRM-MS) in a separate subset of saliva (N = 14). The MRM results of 12 selected proteins confirmed a similar expression pattern as in SWATH-MS analysis. Overall, the results not only demonstrate the longitudinal maternal saliva proteome for the first time but also set the groundwork for comparative analysis between term birth and adverse pregnancy outcomes.
Background: The prevalence of stress and anxiety in diabetes is considerably higher than normal population and found to have a negative impact on diabetes. The aim of this study was to examine the relationships among perceived diabetes-related stress and anxiety behaviors in adults living with type 2 diabetes (T2DM).Methods: In this descriptive and cross sectional study, systematic random sampling technique was used to select the sample of type 2 diabetic patients. Scheduled interview of 412 type 2 diabetic patient was conducted at outpatient department of a tertiary care hospital in New Delhi.Results: Out of 412 diabetic patients, 58.7 percent feel diabetes-related stress and anxiety behaviors when they think about living with diabetes. Age of patients, educational status, occupation and average monthly family income of diabetic patients are statistically associated with perceived diabetes-related stress and anxiety behaviors of type 2 diabetes mellitus patients. Lifestyle behaviour like diet pattern (vegetarian or nonvegetarian diet) and moderate intensity activities like walking are also statistically associated with perceived diabetes-related stress and anxiety behaviors of type 2 diabetes mellitus patients.Conclusions: This study found a high prevalence of diabetes-related stress and anxiety behavior in patients with Type 2 DM. Therefore, the care of individuals with type 2 Diabetes Mellitus (DM) should include the screening and possible management of stress and anxiety in order to achieve and sustain treatment goals.
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