To match the current life-style, there is a huge demand and market for the processed food whose manufacturing requires multiple steps. The mounting demand increases the pressure on the producers and the regulatory bodies to provide sensitive, facile, and cost-effective methods to safeguard consumers’ health. In the multistep process of food processing, there are several chances that the food-spoiling microbes or contaminants could enter the supply chain. In this contest, there is a dire necessity to comprehend, implement, and monitor the levels of contaminants by utilizing various available methods, such as single-cell droplet microfluidic system, DNA biosensor, nanobiosensor, smartphone-based biosensor, aptasensor, and DNA microarray-based methods. The current review focuses on the advancements in these methods for the detection of food-borne contaminants and pathogens.
Stephania glabra is a wild medicinal plant possessing multiple uses as anti-cancer, antimicrobial and antioxidant activities. Different extracts and fractions were prepared from S. glabra tubers and were analyzed for different activities. Evaluation of the total phenolic and flavonoid content revealed that chloroform extract of S. glabra contain maximum phenolic content and maximum flavonoid content was found in methanolic extract. Dry powder analysis of the tubers revealed the presence of saponins, alkaloids and proteins. Ethyl acetate extract of S. glabra tubers showed highest antioxidant potential as revealed by ABTS radical scavenging activity. Methanolic extract has the highest anti-cancer activity while chloroform extracts and fractions have highest antimicrobial activity. These activities can be attributed to the high amount of the phytoconstituents present in these extracts
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