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Objective: The objective of this study was to enrich therapeutic proteins and remove pollutants from dairy wastewater for establishing foam fractionation as a lucrative unit operation. Methods: Dairy wastewater collected from dairy industry was processed to fat-free dried protein waste mass diluted to 1-liter feed by distilled water in different concentrations and foam fractionated by sodium dodecyl sulphate (surfactant) to enriched proteins extract (foamate) in a foam fractionator. Foamate were analysed to quantify total proteins and lacto peroxidase respectively. The efficiency was evaluated by varying parameters like pH, initial waste and ionic concentrations, the waste-surfactant mass ratio of feed and flow rate of gas (N2) through feed solution by several experiments. Heat of desorption (λ) and mass transfer coefficient (K) were determined as indicators of adsorptive bubble separation to foam phase governed by Gibb’s equation of adsorption isotherm. Results: The process was optimized at pH 5.5, initial feed concentration 500μg/ml, waste–surfactant mass ratio (1.5:1), gas flow rate (350 ml/min) and ionic concentration 0.1 gram-mole of NaCL per litre of feed with enrichment factor (49.09), percent recovery (98.18%) observed in foamate. One natural preservative specifically lactoperoxidase was quantified by RP-HPLC analysis as 0.49% (w/w) of total proteins at optimal condition. Heat of desorption(λ), mass transfer coefficient(K)were determined 3140cal/mol and 12.68* 10-9 mol/cal/cm2/s respectively at pH 8.5, initial feed concentration 500μg/ml and gas flow rate 350 ml/min. Conclusion: The method may be a useful unit operation for recovery of biomolecules and removal of toxic pollutants from industrial wastewater for coming days.

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The aim of this study is to enrich therapeutic dairy proteins from 1L of dilute dairy waste by foam fractionation method using anionic surfactant, sodium dodecyl sulphate (SDS) as well as to determine operating parameters of the method like mean bubble diameter, % gas hold up, interfacial area for adsorption, heat of desorption, mass transfer coefficient of adsorption, enrichment ratio (ER) and percentage recovery (%Rp) of proteins to foam phase. The process parameters were optimised by carrying out several experiments and one antineoplastic component namely lactoferrin was quantified in the isolated extract (foamate) by RP-HPLC. The method used 100cm long glass column of internal diameter 8cm and thickness 0.5cm attached with G3 sintered glass sparger(15-40μm) as bubble distributor to feed, rotameter for measurement of gas flow rate (GFR) and N2 gas cylinder as gas supplier. Process parameters like pH and ionic concentration of feed, GFR, initial feed concentration was varied to examine the optimum performance criteria. The result gives maximum enrichment ratio(49.09), %Rp(98.18) of total proteins and 0.98%(w/w) of lactoferrin in foamate at pH (5.5), GFR (350mL/min), initial feed concentration(500μg/mL) , ionic concentration of feed 0.1(M) of NaCL and waste-SDS mass ratio(1.5:1).The heat of desorption(λ) and mass transfer coefficient(K) were estimated at the value of 3140 cal/mol and12.686 * 10-9 mol cal-1 cm-2 s-1 respectively for a specific experiment. It can be concluded that method may be a useful unit operation for enrichment and purification of thermo labile and removal of pollutant proteins from industrial waste water for coming days.