Dyed roots reveal inner complexity
Plant roots do so much more than just hold a plant up. As a site for air storage during flooding, mycorrhizal symbiosis, or carbohydrate storage, the more complex root can tap more complicated functions. Taking advantage of a dye that stains less the deeper it penetrates the tissue, Ortiz-Ramírez
et al
. applied fluorescence-activated cell sorting to the complex cell layers of the maize root. RNA sequencing applied to the single-cell pools defined a developmental map and showed that the mobile transcription factor SHORT-ROOT travels through multiple cell layers and directs this grass root’s anatomical complexity. —PJH
Bambusa vulgaris schrad. Ex j.c. wendl é uma espécie de bambu cultivada em diversas regiões do Brasil. Contudo, há regiões com déficit hídrico pronunciado e o reuso de água é uma importante alternativa com vistas à produção agrícola e florestal. O reuso de efluentes de viveiros de peixes para a irrigação é uma prática agrícola possível desde que haja tolerância da planta a salinidade. Objetivou-se neste trabalho, verificar a tolerância de mudas de B. vulgaris ao estresse hídrico e salino utilizando água residuária da piscicultura na irrigação. Realizado na UFERSA – Mossoró, sob o delineamento experimental em DIC disposto em esquema fatorial 3x5. Foi utilizada água residuária com porcentagem de 0, 10, 15, 20 e 25% de salinidade. Foram analisadas as seguintes variáveis: fitomassa seca da parte aérea e do sistema radicular, concentração de clorofila a, clorofila b e carotenoides, determinação dos teores de macronutrientes, análises de solo e água de irrigação. A variável fitomassa apresentou decréscimo em relação aos demais parâmetros analisados e as mudas de B. vulgaris mostraram mais sensíveis ao estresse hídrico do que à salinidade. Conclui-se que, a água residuária da piscicultura apresenta-se como alternativa para irrigação, entretanto, mudas de Bambusa vulgaris vittata não resistem a falta de irrigação por períodos superiores a 14 dias.
Finding forest species adapted to diff erent soil and climatic conditions and, that provide favorable attributes to commercial use is a challenge. Azadirachta indica is a potential species to attend to this goal. Thus, this paper aims to carry out the vegetative rescue of diff erent Azadirachta indica Juss adult trees by cuttings. For that, Azadirachta indica individuals were randomly selected in a plantation of species set ten years ago and the cut was carried out at 30 cm in height. In sequence, verifi cation was carried out on: (a) the stem cut ability to regrowth (collections and measurements of the shoot length on the 15 th , 30 th , 45 th , and 60 th days); (b) the need to use exogenous auxin for rooting induction [testing 0 (control), 2000, 4000, and 6000 mg.l -1 of Indole-3-butyric acid (IBA)]; (c) the ideal of the propagule size (with 8 cm and 13 cm in height); and (c) the rooting dynamics (every seven days for thirty-fi ve days; the propagules were evaluated for callus formation, oxidation, and rooting). The species showed high regrowth ability, as well as easy rooting with or without the use of IBA; it was found that the ideal size for the propagule is 13 cm and that the process of rooting and root elongation is completed after thirty-fi ve days of staking. The conclusion is: (a) using cuttings for the species vegetative rescue is interesting; (b) 13 cm is considered appropriate for the propagule size, and; (c) the aid of IBA at a dose of 2,000 mg L -1 is ideal to standardize rooting.
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