The research objective was to study and compare promotion behavior for ecotourism of Sub-district Administration Organization (SAO) according to variables of sex, age, education level, family status and tourism. The multi-stage random sampling technique was used for data collecting for 800 SAO members from overall country. The reliability of promotion and conservation of tourism site and regulation control were 0.79 and 0.84 respectively. Data analysis was done with the descriptive statistics of percentage, mean, standard deviation, and inferential statistics with t-test. The results revealed that SAO member had promotion behavior for ecotourism in holistic view at rather high level. The SAO member with different age had different whole promotion behavior for ecotourism with statistically significant at level of 0.05. SAO member with different sex, age had different promotion and conservation of tourism site aspect with statistically significant at level of 0.05, 0.05 and 0.05 respectively. Moreover, SAO member with different education level and family economic status had different regulation control behavior with statistically significant at level of 0.05 and 0.05.
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